School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan.
J Sep Sci. 2011 Apr;34(7):844-51. doi: 10.1002/jssc.201000820. Epub 2011 Mar 4.
Acacia confusa Merr. (Leguminosae), a species native to Taiwan, is widely distributed on the hills and lowlands of Taiwan, and has been used in traditional medicines. In this study, the application of ultrasound-assisted extraction was used to extract the phenolic compounds from A. confusa flowers and buds for the first time. Among the extraction methods, it can significantly enhance the contents of phenolic compounds and antioxidant activities in A. confusa flower and bud extracts using ultrasound-assisted extraction (10 min×12 times). Considering both the solvent consumption and the time needed for extraction, ultrasound-assisted extraction was found to be the most practical approach for the rapid and efficient extraction of bioactive phenolic constituents. In addition, gallic acid, myricitrin-3-rhamnoside, quercitrin-3-rhamnoside, europetin-3-rhamnoside, kaempferol-3-rhamnoside, rhamnetin-3-glucoside, and rhamnetin-3-rhamnoside were also quantified in different extracts by RP-HPLC. It is clear that ultrasound-assisted extraction is an efficient method for extracting phenolic compounds from A. confusa flowers and buds.
台湾相思树（豆科）是台湾特有的一种植物，广泛分布于台湾的丘陵和低地，并被应用于传统药物中。在这项研究中，首次使用超声辅助提取法从台湾相思树的花朵和花蕾中提取酚类化合物。在所有的提取方法中，超声辅助提取（10 分钟×12 次）可以显著提高台湾相思树花朵和花蕾提取物中酚类化合物的含量和抗氧化活性。考虑到溶剂消耗和提取所需的时间，超声辅助提取是一种快速、高效提取生物活性酚类成分的最实用方法。此外，通过反相高效液相色谱法（RP-HPLC）还可以在不同的提取物中定量分析出没食子酸、杨梅素-3-鼠李糖苷、槲皮素-3-鼠李糖苷、圣草酚-3-鼠李糖苷、山奈酚-3-鼠李糖苷、山柰酚-3-葡萄糖苷和山柰酚-3-鼠李糖苷。超声辅助提取是从台湾相思树的花朵和花蕾中提取酚类化合物的一种有效方法。
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