Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Talanta. 2011 Apr 15;84(2):558-64. doi: 10.1016/j.talanta.2011.01.061. Epub 2011 Feb 1.
We have demonstrated the design of a new type fluorescence assay based on the inner filter effect (IFE) of gold nanoparticles (AuNPs) on the fluorescence of quantum dots (QDs). With a high extinction coefficient, AuNPs are expected to be capable of functioning as powerful absorbers. QDs with tunable emission wavelength are ideal fluorophores because the emission spectra of the rationally synthesized QDs can perfectly overlap with the absorption band of the absorber. Aminothiols are chosen as the model analytes, and the IFE-based fluorescent method for detection of aminothiols was suggested. Under the optimum conditions, the response is linearly proportional to the concentration of cysteine in the range of 0.05-0.9 μg mL(-1). The present IFE-based fluorescent strategy could be also used to detect glutathione and homocysteine. The linear concentration ranges were 0.05-1.0 μg mL(-1) for glutathione and 0.01-1.0 μg mL(-1) for homocysteine.
我们展示了一种基于金纳米粒子（AuNPs）对量子点（QDs）荧光的内滤效应（IFE）的新型荧光分析方法的设计。金纳米粒子具有高消光系数，有望成为强大的吸收体。具有可调发射波长的量子点是理想的荧光团，因为合理合成的量子点的发射光谱可以与吸收体的吸收带完全重叠。选择氨基硫醇作为模型分析物，并提出了基于 IFE 的检测氨基硫醇的荧光方法。在最佳条件下，该响应与半胱氨酸的浓度在 0.05-0.9 μg mL(-1)范围内呈线性比例关系。本基于 IFE 的荧光策略也可用于检测谷胱甘肽和同型半胱氨酸。谷胱甘肽的线性浓度范围为 0.05-1.0 μg mL(-1)，同型半胱氨酸的线性浓度范围为 0.01-1.0 μg mL(-1)。
