分子印迹固相萃取-高效毛细管电泳法测定鸡肉中恩诺沙星残留量
[Determination of enrofloxacin residue in chicken muscle using molecular imprinted solid phase extraction-high performance capillary electrophoresis].
作者信息
Wang Xueyan, Tan Huarong, Qi Kezong, Shao Li, Li Hui, Xue Xiuheng, Xie Yin
机构信息
College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China.
出版信息
Se Pu. 2010 Nov;28(11):1107-10.
Molecular imprinted polymer targeted for enrofloxacin (ENR) was synthesized using ENR as the template molecules, alpha-methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking agent. With the ENR molecular imprinted polymer, a molecular imprinted solid phase extraction-high performance capillary electrophoresis (MISPE-HPCE) method for the determination of ENR in chicken muscle was developed. The results showed that this method exhibited high selectivity and sensitivity for the determination of ENR in chicken muscle. Under the optimized conditions, the limit of detection and limit of quantification were 92.02 microg/kg and 336.04 microg/kg, respectively. The recoveries of the ENR spiked in chicken muscle at different levels were in the range of 77.84%-86.52%, and the relative standard deviations (RSDs) were in the range of 2.18%-3.76%. This MISPE-HPCE method is feasible for the analysis of ENR residue in chicken muscle.
以恩诺沙星（ENR）为模板分子、α-甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂，合成了针对恩诺沙星的分子印迹聚合物。利用该恩诺沙星分子印迹聚合物，建立了一种用于测定鸡肉中恩诺沙星的分子印迹固相萃取-高效毛细管电泳（MISPE-HPCE）方法。结果表明，该方法对鸡肉中恩诺沙星的测定具有高选择性和高灵敏度。在优化条件下，检测限和定量限分别为92.02μg/kg和336.04μg/kg。不同添加水平的恩诺沙星在鸡肉中的回收率在77.84% - 86.52%范围内，相对标准偏差（RSD）在2.18% - 3.76%范围内。该MISPE-HPCE方法用于分析鸡肉中恩诺沙星残留是可行的。
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