用于测定放线菌素-D浓度的流式细胞术竞争性结合测定法。
Flow cytometric competitive binding assay for determination of actinomycin-D concentrations.
作者信息
Saunders G C, Martin J C, Jett J H, Perkins A
机构信息
Cell Biology Group, Los Alamos National Laboratory, New Mexico 87545.
出版信息
Cytometry. 1990;11(2):311-3. doi: 10.1002/cyto.990110213.
PMID:2138531
Abstract
A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycin-D for common binding sites on DNA coated 10 microns diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the presence of a constant amount of mithramycin, the microsphere fluorescence is inversely proportional to actinomycin-D concentration.
摘要
本文描述了一种在直径为10微米的包被有DNA的微球上，荧光抗生素光神霉素和放线菌素-D针对共同结合位点的单步、无需分离的竞争性结合反应。用流式细胞仪测量微球的荧光。在光神霉素量恒定的情况下，微球荧光与放线菌素-D浓度成反比。
Zotero 插件