意大利患者的 Merkel 细胞癌中的 Merkel 细胞多瘤病毒。
Merkel cell polyomavirus in Merkel cell carcinoma of Italian patients.
机构信息
Laboratory of Virology, Regina Elena Cancer Institute Via delle Messi d'Oro 156 00158 Rome, Italy.
出版信息
Virol J. 2011 Mar 7;8:103. doi: 10.1186/1743-422X-8-103.
BACKGROUND
Merkel cell carcinoma (MCC) is a rare but very aggressive human malignancy of elderly or immunosuppressed patients. Clonal integration of a new human polyomavirus, the Merkel cell polyomavirus (MCPyV), has been reported in MCC patients. The main objective of the study was the detection of MCPyV and viral expression in clinical samples of Italian patients who were diagnosed MCC.
FINDINGS
DNA and RNA were extracted from nine MCCs to detect the presence of MCPyV. Viral large T gene (LT1 and LT3), and viral capsid gene (VP1) were detected by polymerase chain reaction (PCR) based methods, and the amplified PCR products were subjected to direct sequencing. The presence of viral T antigen and/or viral capsid DNA sequences was demonstrated in eight of the nine MCC lesions, whereas RNA transcripts were detected in three MCCs.
CONCLUSIONS
These findings indicate a potential role of MCPyV in the pathogenesis of at least a subset of MCCs.
背景
默克尔细胞癌（MCC）是一种罕见但非常侵袭性的人类恶性肿瘤，发生于老年或免疫抑制患者。在 MCC 患者中已报道了一种新的人类多瘤病毒，即默克尔细胞多瘤病毒（MCPyV）的克隆整合。本研究的主要目的是检测意大利 MCC 患者临床样本中 MCPyV 的存在及其病毒表达情况。
发现
从 9 例 MCC 中提取 DNA 和 RNA，以检测 MCPyV 的存在。采用基于聚合酶链反应（PCR）的方法检测病毒大 T 基因（LT1 和 LT3）和病毒衣壳基因（VP1），并对扩增的 PCR 产物进行直接测序。在 9 例 MCC 病变中有 8 例证实存在病毒 T 抗原和/或病毒衣壳 DNA 序列，而在 3 例 MCC 中检测到 RNA 转录本。
结论
这些发现表明 MCPyV 在至少一部分 MCC 的发病机制中可能起作用。
Zotero 插件