Kannan M Mari, Quine S Darlin
SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu, India; Department of Pharmacology, Jayamukhi College of Pharmacy, Narsampet, Warangal, Andhra Pradesh, India.
Post Graduate and Research Department of Chemistry, Government Arts College, C.Mutlur, Chidambaram, Tamil Nadu, India.
Eur J Pharmacol. 2011 May 20;659(1):45-52. doi: 10.1016/j.ejphar.2011.02.037. Epub 2011 Mar 5.
The present study was designed to evaluate the cardioprotective effects of ellagic acid against isoproterenol induced myocardial infarction in rats by studying electrocardiography, blood pressure, cardiac markers, lipid peroxidation, antioxidant defense system and histological changes. Male Wistar rats were treated orally with ellagic acid (7.5 and 15mg/kg) daily for a period of 10 days. After 10 days of pretreatment, isoproterenol (100mg/kg) was injected subcutaneously to rats at an interval of 24h for 2 days to induce myocardial infarction. Isoproterenol administered rats showed significant changes in the electrocardiogram pattern, arterial pressure, and heart rate. Isoproterenol-induced rats also showed significant (P<0.05) increase in the levels of serum troponin-I, creatine kinase, lactate dehydrogenase, C-reactive protein, plasma homocysteine, heart tissue thiobarbituric acid reactive substances and lipid hydro peroxides. The activities/levels of antioxidant system were decreased in isoproterenol-induced rats. The histopathological findings of the myocardial tissue evidenced myocardial damage in isoproterenol induced rats. The oral pretreatment of ellagic acid restored the pathological electrocardiographic patterns, regulated the arterial blood pressures and heart rate in the isoproterenol induced myocardial infarcted rats. The ellagic acid pretreatment significantly reduced the levels of biochemical markers, lipid peroxidation and significantly increased the activities/levels of the antioxidant system in the isoproterenol induced rats. An inhibited myocardial necrosis was evidenced by the histopathological findings in ellagic acid pretreated isoproterenol induced rats. Our study shows that oral pretreatment of ellagic acid prevents isoproterenol induced oxidative stress in myocardial infarction.
本研究旨在通过研究心电图、血压、心脏标志物、脂质过氧化、抗氧化防御系统和组织学变化，评估鞣花酸对异丙肾上腺素诱导的大鼠心肌梗死的心脏保护作用。雄性Wistar大鼠每天口服鞣花酸（7.5和15mg/kg），持续10天。预处理10天后，每隔24小时皮下注射异丙肾上腺素（100mg/kg）给大鼠，连续2天以诱导心肌梗死。给予异丙肾上腺素的大鼠在心电图模式、动脉压和心率方面表现出显著变化。异丙肾上腺素诱导的大鼠血清肌钙蛋白-I、肌酸激酶、乳酸脱氢酶、C反应蛋白、血浆同型半胱氨酸、心脏组织硫代巴比妥酸反应物质和脂质氢过氧化物水平也显著（P<0.05）升高。异丙肾上腺素诱导的大鼠抗氧化系统的活性/水平降低。心肌组织的组织病理学结果证明异丙肾上腺素诱导的大鼠存在心肌损伤。鞣花酸的口服预处理恢复了异丙肾上腺素诱导的心肌梗死大鼠的病理性心电图模式，调节了动脉血压和心率。鞣花酸预处理显著降低了异丙肾上腺素诱导的大鼠生化标志物水平、脂质过氧化，并显著提高了抗氧化系统的活性/水平。鞣花酸预处理的异丙肾上腺素诱导的大鼠的组织病理学结果证明心肌坏死受到抑制。我们的研究表明，鞣花酸的口服预处理可预防异丙肾上腺素诱导的心肌梗死中的氧化应激。
