School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
Nutrition. 2011 Apr;27(4):463-70. doi: 10.1016/j.nut.2010.09.006.
This study investigated the effects of indole-3-carbinol (I3C), a cruciferous vegetable derivative, on obesity and its associated factors in high-fat-diet-induced obese (DIO) mice.
Eighteen male C57BL/6 mice were randomly assigned to one of three groups: basal, high fat (HF), and HF + 5 mg/kg of I3C intraperitoneally (HFI). After 12 wk of treatment, obesity-associated factors, including body weight, organ weight, serum concentrations of glucose, triacylglycerol, insulin, and adipokines, and macrophage accumulation and lipid metabolism-associated factors in epididymal adipose tissue were measured.
Body weight and epididymal adipose tissue weight were greater (P < 0.01), and adipocytes were larger in the HF group than in the basal and HFI groups. Compared with the HF group, the HFI group had improved glucose tolerance, a higher serum adiponectin concentration, lower serum glucose, triacylglycerol, insulin, and leptin concentrations, and less F4/80 expression in epididymal adipose tissue (P < 0.001). Furthermore, I3C treatment decreased acetyl coenzyme A carboxylase mRNA expression (P < 0.05) and increased peroxisome proliferator-activated receptor-γ protein expression (P < 0.05) in epididymal adipose tissue of DIO mice.
The I3C treatment decreased body weight and fat accumulation and infiltrated macrophages in epididymal adipose tissue of DIO mice, and these reductions were associated with improved glucose tolerance and with modulated expression of adipokines and lipogenic-associated gene products, including acetyl coenzyme A carboxylase and peroxisome proliferator-activated receptor-γ.
本研究旨在探讨吲哚-3-甲醇（I3C），一种十字花科蔬菜衍生物，对高脂肪饮食诱导肥胖（DIO）小鼠肥胖及其相关因素的影响。
将 18 只雄性 C57BL/6 小鼠随机分为三组：基础组、高脂肪组（HF）和腹腔内给予 5mg/kg I3C 的高脂肪组（HFI）。治疗 12 周后，测量肥胖相关因素，包括体重、器官重量、血清葡萄糖、三酰甘油、胰岛素和脂肪因子浓度，以及附睾脂肪组织中巨噬细胞积累和脂质代谢相关因素。
与基础组和 HFI 组相比，HF 组体重和附睾脂肪组织重量更大（P < 0.01），脂肪细胞更大。与 HF 组相比，HFI 组葡萄糖耐量改善，血清脂联素浓度更高，血清葡萄糖、三酰甘油、胰岛素和瘦素浓度更低，附睾脂肪组织中 F4/80 表达更少（P < 0.001）。此外，I3C 处理降低了 DIO 小鼠附睾脂肪组织中乙酰辅酶 A 羧化酶 mRNA 表达（P < 0.05），并增加了过氧化物酶体增殖物激活受体-γ 蛋白表达（P < 0.05）。
I3C 处理可降低 DIO 小鼠体重和脂肪堆积，并减少附睾脂肪组织中浸润的巨噬细胞，这些减少与改善葡萄糖耐量以及调节脂肪因子和脂肪生成相关基因产物的表达有关，包括乙酰辅酶 A 羧化酶和过氧化物酶体增殖物激活受体-γ。
