Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.
Blood. 2011 May 5;117(18):4773-7. doi: 10.1182/blood-2011-01-330514. Epub 2011 Mar 10.
Cryopreservation of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) is crucial for cord blood (CB) banking and transplantation. We evaluated recovery of functional HPC cryopreserved as mononuclear or unseparated cells for up to 23.5 years compared with prefreeze values of the same CB units. Highly efficient recovery (80%-100%) was apparent for granulocyte-macrophage and multipotential hematopoietic progenitors, although some collections had reproducible low recovery. Proliferative potential, response to multiple cytokines, and replating of HPC colonies was extensive. CD34(+) cells isolated from CB cryopreserved for up to 21 years had long-term (≥ 6 month) engrafting capability in primary and secondary immunodeficient mice reflecting recovery of long-term repopulating, self-renewing HSCs. We recovered functionally responsive CD4(+) and CD8(+) T lymphocytes, generated induced pluripotent stem (iPS) cells with differentiation representing all 3 germ cell lineages in vitro and in vivo, and detected high proliferative endothelial colony forming cells, results of relevance to CB biology and banking.
造血干细胞（HSCs）和造血祖细胞（HPCs）的冷冻保存对于脐带血（CB）库和移植至关重要。我们评估了单核或未分离细胞冷冻保存的功能性 HPC 的恢复情况，与同一 CB 单位的冷冻前值相比，最长可达 23.5 年。尽管某些采集物的恢复率可重复出现较低，但粒细胞-巨噬细胞和多能造血祖细胞的恢复效率非常高（80%-100%）。HPC 集落的增殖潜力、对多种细胞因子的反应和再种植非常广泛。从冷冻保存长达 21 年的 CB 中分离的 CD34+细胞在原发性和继发性免疫缺陷小鼠中具有长期（≥6 个月）植入能力，反映了长期重建成体、自我更新 HSCs 的恢复。我们恢复了功能反应性的 CD4+和 CD8+T 淋巴细胞，体外和体内产生了具有分化潜能的诱导多能干细胞（iPS）细胞，代表了所有 3 个生殖细胞谱系，并且检测到了高增殖性的内皮细胞集落形成细胞，这些结果与 CB 生物学和储存有关。
