Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB), CRC Corporació Sanitària, Barcelona, Spain.
Neuroscience. 2011 May 19;182:208-16. doi: 10.1016/j.neuroscience.2011.03.013. Epub 2011 Mar 21.
Positron emission tomography (PET) studies in humans have used (11)C-flumazenil (FMZ) to assess neuronal viability after stroke. Here we aimed to study whether (11)C-FMZ binding was sensitive to neuronal damage in the acute phase following ischemia/reperfusion in the rat brain.
Transient (2 h followed by reperfusion) and permanent intraluminal middle cerebral artery occlusion was carried out. (11)C-FMZ binding was studied by PET up to 24 h after the onset of ischemia. Tissue infarction was evaluated post-mortem at 24 h. Immunohistochemistry against a neuronal nuclei specific protein (NeuN) was performed to assess neuronal injury.
No decrease in (11)C-FMZ binding was detected in the ipsilateral cortex up to 24 h post-ischemia in the model of transient occlusion despite the fact that rats developed cortical and striatal infarction, and neuronal injury was clearly apparent at this time. In contrast, (11)C-FMZ binding was significantly depressed in the ipsilateral cortex at 24 h following permanent ischemia.
This finding evidences that (11)C-FMZ binding is not sensitive to neuronal damage on the acute phase of ischemia/reperfusion in the rat brain.
正电子发射断层扫描（PET）研究已经使用（11）C-氟马西尼（FMZ）来评估中风后神经元的存活情况。在此，我们旨在研究在大鼠脑缺血/再灌注后的急性期，（11）C-FMZ 结合是否对神经元损伤敏感。
进行短暂（2 小时后再灌注）和永久性管腔内大脑中动脉闭塞。在缺血后 24 小时内通过 PET 研究（11）C-FMZ 结合。在 24 小时后进行死后组织梗死评估。进行神经元核特异性蛋白（NeuN）的免疫组织化学染色以评估神经元损伤。
在短暂闭塞模型中，即使大鼠发生皮质和纹状体梗死，并且此时神经元损伤明显，（11）C-FMZ 结合在缺血后 24 小时内也未检测到对侧皮质的减少。相比之下，在永久性缺血后 24 小时，（11）C-FMZ 结合在对侧皮质显著降低。
这一发现表明，（11）C-FMZ 结合对大鼠脑缺血/再灌注的急性期神经元损伤不敏感。
