Yui Hitoshi, Imaizumi Uno, Beppu Hisashi, Ito Mitsuhiro, Furuya Munetaka, Arisaka Hirofumi, Yoshida Kazu-Ichi
Division of Anesthesiology, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaokacho, Yokosuka-shi, Kanagawa 238-8580, Japan.
Anesthesiol Res Pract. 2011;2011:521084. doi: 10.1155/2011/521084. Epub 2011 Mar 2.
The aim of this experiment was to establish whether verapamil, nicardipine, and nitroglycerin have (1) infarct size-limiting effects and (2) antiarrhythmic effects in in vivo rabbit hearts during ischemia/reperfusion. Rabbits received regional ischemia by 30 min of left anterior descending coronary artery occlusion followed by 3 hours of reperfusion under ketamine and xylazine anesthesia. The animals were randomly assigned to the following 4 treatment groups: a control group, a verapamil group, a nicardipine group, and a nitroglycerin group. A continuous infusion of verapamil, nicardipine, or nitroglycerin was initiated 5 min prior to ischemia. Infarct size/area at risk decreased in verapamil, and nitroglycerin. The incidence of ischemia-induced arrhythmia decreased in nicardipine, verapamil and nitroglycerin. The incidence of reperfusion-induced arrhythmias decreased in verapamil and nitroglycerin. From the present experimental results, verapamil and nitroglycerin rather than nicardipine did afford significant protection to the heart subjected to ischemia and reperfusion in a rabbit model.
本实验的目的是确定维拉帕米、尼卡地平及硝酸甘油在兔心脏缺血/再灌注的体内实验中是否具有：（1）限制梗死面积的作用；（2）抗心律失常作用。兔在氯胺酮和赛拉嗪麻醉下，左冠状动脉前降支闭塞30分钟造成局部缺血，随后再灌注3小时。动物被随机分为以下4个治疗组：对照组、维拉帕米组、尼卡地平组和硝酸甘油组。在缺血前5分钟开始持续输注维拉帕米、尼卡地平或硝酸甘油。维拉帕米组和硝酸甘油组梗死面积/危险区面积减小。尼卡地平组、维拉帕米组和硝酸甘油组缺血诱导的心律失常发生率降低。维拉帕米组和硝酸甘油组再灌注诱导的心律失常发生率降低。从目前的实验结果来看，在兔模型中，维拉帕米和硝酸甘油而非尼卡地平确实对遭受缺血和再灌注的心脏提供了显著保护。
