利用 IκB-EGFP 嵌合基因作为指标鉴定具有 NF-κB 抑制剂活性的微生物代谢产物。

Utilization of IκB-EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity.

机构信息

Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.

Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan.

出版信息

Biol Proced Online. 2010 Oct 2;12(1):131-138. doi: 10.1007/s12575-010-9033-9.

DOI:10.1007/s12575-010-9033-9

PMID:21406073

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3055915/

Abstract

NF-κB regulates several important expressions, such as cytokine release, anti-apoptosis, adhesion molecule expression, and cell cycle processing. Several NF-κB inhibitors have been discovered as an anti-tumor or anti-inflammatory drug. The activity of NF-κB transcription factor is negatively regulated by IκB binding. In this study, IκB assay system was established and IκB-EGFP fusion protein was used as an indicator to monitor the effects of substances on the IκB degradation. The results indicated that the chosen hydroquinone could inhibit the IκB degradation and cause the cell de-attachment from the bottom of culture plate. In addition, this system could also monitor the IκB degradation of microbial metabolite of natural mixtures of propolis. Thus, the IκB assay system may be a good system for drug discovery related to microbial metabolite.

摘要

NF-κB 调节几种重要的表达，如细胞因子释放、抗凋亡、黏附分子表达和细胞周期处理。已经发现几种 NF-κB 抑制剂可用作抗肿瘤或抗炎药物。NF-κB 转录因子的活性受 IκB 结合的负调控。在这项研究中，建立了 IκB 测定系统，并使用 IκB-EGFP 融合蛋白作为指标来监测物质对 IκB 降解的影响。结果表明，所选的对苯二酚能够抑制 IκB 降解并导致细胞从培养板底部脱落。此外，该系统还可以监测天然蜂胶混合物的微生物代谢产物的 IκB 降解。因此，IκB 测定系统可能是与微生物代谢产物相关的药物发现的良好系统。