Purification and properties of bovine liver lysosomal adenosine 5'-phosphosulphate sulphohydrolase. A non-specific enzyme with pyrophosphatase and phosphodiesterase activities.

Bovine liver lysosomal adenosine 5'-phosphosulphate sulphohydrolase (EC 3.6.2.1) was purified to apparent homogeneity. The molecular weight of the enzyme was 53 000 by sodium dodecyl sulphate polyacrylamide gel electrophoresis and 56 000 by BioGel P-150 gel filtration. The substrate specificity of the enzyme was studied. The several substrates towards which the enzyme preparation showed high activity were used to establish that a single enzyme was responsible for the different activities. This multiple specificity provides a possible explanation of the physiological role of lysosomal adenosine 5'-phosphosulphate sulphohydrolase.