Li Qin, Kong Ping, Wu Cai-Xia, Sun Chong-Wei, Chen Yan-Lin, Xu Xiao-Qun, Zhang Da-Liang
Department of Otorhinolaryngology, Linyi People's Hospital of Shandong Province, Linyi 276003, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 Jan;46(1):59-62.
To investigate the effects of intranasal interferon gamma (IFN-γ) on nasal mucosa remodeling and expression of transforming growth factor-β1 (TGF-β1), Smad2, Smad3, Smad7 in allergic rhinitis (AR) rat model.
Ovalbumin (OVA) and aluminum hydroxide were used to construct the AR model. Thirty AR rats were randomly divided into positive control group (group B, n = 10), IFN-γ treatment group (group C, n = 10) and negative control group (normal rats, n = 10). After the AR models were built, 50 µl PBS, 1 µg IFN-γ was dropped into the nasal cavity of each rat in group B and group C, from the fouth week to tenth week, twice a week. The nasal mucosa was collected on day 71 in order to observe the pathologic changes, and the expression of TGF-β1, TGF-β1 mRNA, Smad2 mRNA, Smad3 mRNA and Smad7 mRNA by immunohistochemistry and reverse transcriptase-polymerase chain reaction.
Decreases of TGF-β1, Smad2 and Smad3 mRNA were seen in nasal tissue of group C (0.59 ± 0.04, 0.39 ± 0.08, 0.46 ± 0.15) as compared with group B (0.82 ± 0.12, 0.70 ± 0.18, 0.95 ± 0.26), the differences were significant (q value were 3.15, 4.47, 3.03, all P < 0.05). The levels of Smad7 mRNA expression increased significantly (q = 2.98, P < 0.05) in group C (0.31 ± 0.05) as compared with group B (0.25 ± 0.06). Immunohistochemistry showed significant decrease of TGF-β1 expression in the nasal tissue of group C much lesser than that in group B.
Intranasal IFN-γ could decrease the expression of TGF-β1, TGF-β1 mRNA, Smad2 mRNA, Smad3 mRNA, increase the expression of Smad7 mRNA in AR rats model and inhibit the nasal mucosa remodeling.
探讨鼻内注射干扰素γ（IFN-γ）对变应性鼻炎（AR）大鼠模型鼻黏膜重塑及转化生长因子-β1（TGF-β1）、Smad2、Smad3、Smad7表达的影响。
用卵清蛋白（OVA）和氢氧化铝构建AR模型。30只AR大鼠随机分为阳性对照组（B组，n = 10）、IFN-γ治疗组（C组，n = 10）和阴性对照组（正常大鼠，n = 10）。AR模型建立后，B组和C组每只大鼠鼻腔滴入50 μl PBS、1 μg IFN-γ，从第4周持续至第10周，每周2次。于第71天采集鼻黏膜，观察其病理变化，并通过免疫组织化学和逆转录聚合酶链反应检测TGF-β1、TGF-β1 mRNA、Smad2 mRNA、Smad3 mRNA和Smad7 mRNA的表达。
与B组（0.82±0.12、0.70±0.18、0.95±0.26）相比，C组鼻组织中TGF-β1、Smad2和Smad3 mRNA表达降低（分别为0.59±0.04、0.39±0.08、0.46±0.15），差异有统计学意义（q值分别为3.15、4.47、3.03，均P<0.05）。与B组（0.25±0.06）相比，C组Smad7 mRNA表达水平显著升高（q = 2.98，P<0.05）（0.31±0.05）。免疫组织化学显示，C组鼻组织中TGF-β1表达显著低于B组。
鼻内注射IFN-γ可降低AR大鼠模型中TGF-β1、TGF-β1 mRNA、Smad2 mRNA、Smad3 mRNA的表达，增加Smad7 mRNA的表达，并抑制鼻黏膜重塑。
Zotero 插件