Hamm J, Darzynkiewicz E, Tahara S M, Mattaj I W
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Cell. 1990 Aug 10;62(3):569-77. doi: 10.1016/0092-8674(90)90021-6.
The ability of series of U1 snRNAs and U6 snRNAs to migrate into the nucleus of Xenopus oocytes after injection into the cytoplasm was analyzed. The U snRNAs were made either by injecting U snRNA genes into the nucleus of oocytes or, synthetically, by T7 RNA polymerase, incorporating a variety of cap structures. The results indicate that nuclear targeting of U1 snRNA requires both a trimethylguanosine cap structure and binding of at least one common U snRNP protein. Using synthetic U6 snRNAs, it is further demonstrated that the trimethylguanosine cap structure can act in nuclear targeting in the absence of the common U snRNP proteins. These results imply that U snRNP nuclear targeting signals are of a modular nature.
分析了一系列U1 snRNA和U6 snRNA注入爪蟾卵母细胞细胞质后迁移到细胞核的能力。U snRNA可通过将U snRNA基因注入卵母细胞核内产生，也可通过T7 RNA聚合酶合成产生，并带有多种帽结构。结果表明，U1 snRNA的核靶向需要三甲基鸟苷帽结构以及至少一种常见U snRNP蛋白的结合。使用合成的U6 snRNA进一步证明，在没有常见U snRNP蛋白的情况下，三甲基鸟苷帽结构可在核靶向中发挥作用。这些结果表明，U snRNP核靶向信号具有模块化性质。
