Kredo-Russo Sharon, Hornstein Eran
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Methods Mol Biol. 2011;732:89-97. doi: 10.1007/978-1-61779-083-6_7.
Here, we detail a protocol to design and introduce sequence-specific cholesterol-conjugated antisense oligonucleotides into mouse organ culture. We review design principles for "antagomirs", antisense oligos with a cholesterol-moiety modification at the 3', and present an optimized method to apply them onto 3D cultured embryonic pancreas. The method offers an approach to study the developmental functions of individual miRNAs and to evaluate miRNA targets, which is significantly faster and simpler than comparable genetics-based approaches.
在此，我们详细介绍一种将序列特异性胆固醇缀合反义寡核苷酸设计并引入小鼠器官培养的方案。我们回顾了“抗微小RNA（antagomirs）”（3' 端带有胆固醇部分修饰的反义寡核苷酸）的设计原则，并提出一种将其应用于三维培养胚胎胰腺的优化方法。该方法提供了一种研究单个微小RNA（miRNA）发育功能以及评估miRNA靶标的途径，比基于遗传学的类似方法显著更快且更简单。
