Moss J, Woodrow D F, Shore I, Gower P, Phillips M, Spiro R G
Department of Histopathology, Charing Cross and Westminster Medical School, London, U.K.
J Pathol. 1990 Jun;161(2):137-43. doi: 10.1002/path.1711610208.
The distribution of heparan sulphate proteoglycans (HSPG) has been investigated in normal human glomeruli, membranous glomerulonephritis, mesangial IgA disease, and anti-glomerular basement membrane disease. HSPG was localized using anti-bovine HSPG antibody and 10 nm gold-labelled secondary antibody on paraformaldehyde-fixed, Lowicryl K4M resin-embedded kidneys. HSPG was present in all glomeruli and there was a zonation of its distribution in that it was predominantly on the epithelial aspect of the glomerular basement membrane (GBM) and mesangium with little in the central regions of the mesangial matrix. In the cases of immune complex glomerulonephritis, no HSPG was found in the electron-dense deposits. These findings contrast with our previous studies using the same technique in which type IV collagen and fibronectin were found predominantly on the endothelial aspect of the GBM.
已对正常人类肾小球、膜性肾小球肾炎、系膜IgA病和抗肾小球基底膜病中硫酸乙酰肝素蛋白聚糖（HSPG）的分布进行了研究。使用抗牛HSPG抗体和10纳米金标记二抗，对经多聚甲醛固定、用Lowicryl K4M树脂包埋的肾脏进行HSPG定位。HSPG存在于所有肾小球中，其分布呈分区状态，主要位于肾小球基底膜（GBM）和系膜的上皮侧，系膜基质中央区域含量很少。在免疫复合物性肾小球肾炎病例中，在电子致密沉积物中未发现HSPG。这些发现与我们之前使用相同技术的研究结果形成对比，在之前的研究中，IV型胶原和纤连蛋白主要位于GBM的内皮侧。
