Eriksen K, Landsverk T, Bodahl E G
Department of Pathology, Norwegian College of Veterinary Medicine, Oslo.
Res Vet Sci. 1990 Jul;49(1):1-7.
The distribution of enzymes and laminin was examined in ileal tissue from pigs suffering from intestinal adenomatosis to reveal the nature of the lesion. A disruption of the normal and specific pattern of distribution was found. Thus, the normal ileal epithelium was characterised by brush border enzymes: alkaline phosphatase, magnesium-dependent adenosine triphosphatase (Mg-ATPase), fluoride resistant acid phosphatase and 5'-nucleotidase; enzymes of the basolateral border: Mg-ATPase; and cytoplasmic enzymes: beta-glucuronidase, non-specific esterase and acid phosphatase. Subepithelial fibroblasts seemed to be characterised by 5'-nucleotidase. Laminin was present as a continuous band under the surface and crypt epithelium, somewhat thicker in the former. In contrast, the branching proliferating crypts of intestinal adenomatosis largely lacked enzymes characteristic of both villus and crypt cells. Reactions for the subepithelial components, laminin and fibroblasts were also reduced. The deficient differentiation of the epithelial as well as subepithelial components in porcine intestinal adenomatosis distinguish the condition from crypt hyperplasia and indicate an adenoma-like character.
对患有肠道腺瘤病的猪的回肠组织进行了酶和层粘连蛋白分布的检测，以揭示病变的本质。发现正常和特定的分布模式遭到破坏。因此，正常回肠上皮的特征在于刷状缘酶：碱性磷酸酶、镁依赖性三磷酸腺苷酶（Mg-ATPase）、耐氟酸性磷酸酶和5'-核苷酸酶；基底外侧缘酶：Mg-ATPase；以及细胞质酶：β-葡萄糖醛酸酶、非特异性酯酶和酸性磷酸酶。上皮下成纤维细胞似乎以5'-核苷酸酶为特征。层粘连蛋白在表面和隐窝上皮下呈连续带，在前者中稍厚。相比之下，肠道腺瘤病的分支增生性隐窝在很大程度上缺乏绒毛和隐窝细胞特有的酶。上皮下成分、层粘连蛋白和成纤维细胞的反应也减弱。猪肠道腺瘤病中上皮和上皮下成分的分化不足将该病症与隐窝增生区分开来，并表明其具有腺瘤样特征。
