佐剂关节炎影响管家基因的表达。
Adjuvant arthritis influences expression of housekeeping genes.
机构信息
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2N8, Canada.
出版信息
Inflamm Res. 2011 Jun;60(6):521-3. doi: 10.1007/s00011-011-0327-4. Epub 2011 Mar 25.
OBJECTIVE AND METHOD
We determined whether adjuvant arthritis (AA) affects expression of three housekeeping (HK) genes, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), β-actin and 18s ribosomal RNA (18s rRNA) in organs of Sprague-Dawley rats using the real-time reverse-transcription polymerase chain reaction (RT-PCR).
RESULTS
Expression of 18s rRNA was unaffected by AA whereas that of β-actin was reduced by 52, 62 and 81% in the liver, kidney and intestine, respectively, but not in the heart. AA significantly reduced GAPDH expression in the intestine.
CONCLUSION
For reliable results, the HK gene must be carefully selected.
目的与方法
我们通过实时逆转录聚合酶链反应（RT-PCR）确定佐剂性关节炎（AA）是否影响 Sprague-Dawley 大鼠器官中三种管家（HK）基因，即甘油醛-3-磷酸脱氢酶（GAPDH）、β-肌动蛋白和 18s 核糖体 RNA（18s rRNA）的表达。
结果
AA 对 18s rRNA 的表达没有影响，但分别使肝脏、肾脏和肠道中β-肌动蛋白的表达降低了 52%、62%和 81%，而心脏中没有变化。AA 显著降低了肠道中 GAPDH 的表达。
结论
为了获得可靠的结果，必须仔细选择 HK 基因。
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