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葱属植物栽培植株与组织培养器官抗氧化状态的比较研究。

Comparative study on Allium schoenoprasum cultivated plant and Allium schoenoprasum tissue culture organs antioxidant status.

机构信息

Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Yugoslavia.

出版信息

Phytother Res. 2011 Nov;25(11):1618-22. doi: 10.1002/ptr.3394. Epub 2011 Mar 17.

Abstract

This study was designed to examine Allium schoenoprasum tissue culture organs antioxidant and scavenging activity and to make a comparison between Allium schoenoprasum cultivated plant and Allium schoenoprasum tissue culture organs antioxidant activity. This study reports the results on the root, stalk and leaf antioxidant enzyme activities (superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase), reduced glutathione quantity, flavonoids and soluble protein contents and quantities of malonyldialdehyde and ·OH radical. In Allium schoenoprasum tissue culture organs the total antioxidant capacity was determined by the FRAP method and scavenger activity by the DPPH method. The present results indicated that the crude extract of Allium schoenoprasum tissue culture exhibited antioxidant and scavenging abilities in all investigated plant parts, especially in the roots. According to our results, the tissue culture plants exhibited the highest activities in the roots in contrast to the cultivated plants where highest activities were observed in the leaves.

摘要

本研究旨在检测葱组织培养器官的抗氧化和清除活性,并比较栽培葱和葱组织培养器官的抗氧化活性。本研究报告了根、茎和叶抗氧化酶活性(超氧化物歧化酶、过氧化氢酶、愈创木酚过氧化物酶和谷胱甘肽过氧化物酶)、还原型谷胱甘肽含量、类黄酮和可溶性蛋白质含量以及丙二醛和·OH 自由基含量的结果。在葱组织培养器官中,总抗氧化能力通过 FRAP 法测定,清除活性通过 DPPH 法测定。目前的结果表明,葱组织培养的粗提取物在所有研究的植物部分都表现出抗氧化和清除能力,特别是在根部。根据我们的结果,组织培养植物在根部表现出最高的活性,而在栽培植物中,叶片表现出最高的活性。

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