PI3K/Akt signaling pathway is involved in the pathogenesis of ulcerative colitis.

OBJECTIVE

The purpose of this study was to investigate the role of the PI3K/Akt signaling pathway in the pathogenesis of ulcerative colitis (UC).

MATERIALS AND METHODS

Mucosal biopsy specimens from 54 active UC cases and adjacent normal tissues from 18 colon cancer cases were investigated. Twenty-eight Balb/c mice were randomly divided into four groups. Dextran sulphate sodium (DSS) solution (5%) was used to develop the mouse colitis model. After treatment with wortmannin (a PI3K inhibitor), disease activity index (DAI) and histological score was determined for each group of mice. Expression of phosphorylated Akt (p-Akt) in UC patients and mouse intestinal mucosa was determined by immunohistochemical staining. We also determined the effect of wortmannin on tumor necrosis factor-α (TNF-α) expression in intestinal biopsy tissues of UC patients and mice with DSS-induced colitis.

RESULTS

Wortmannin significantly reduced the level of p-Akt and TNF-α in the colitis tissues of UC patients and DSS-treated mice. Wortmannin significantly alleviated the inflammation of colitis as assessed by DAI and histological score in DSS-treated mice.

CONCLUSION

The PI3K/Akt signal transduction pathway is involved in the regulation and release of pro-inflammatory cytokines such as TNF-α and plays an important role in the development and progression of UC.