Su Shi-ming, Zeng Xi-bai, Jiang Xi-liang, Bai Ling-yu, Li Lian-fang, Zhang Yan-rong
Ministry ofAgriculture Key Laboratory of Agro-Environment & Climate Change, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Ying Yong Sheng Tai Xue Bao. 2010 Dec;21(12):3225-30.
A total of thirteen fungal strains with higher arsenic (As)-tolerance ability were isolated from six As-contaminated soil samples collected from the mining areas of Shimen County and Chenz-hou City in Hunan Province. Among the strains isolated, Penicillin janthinellum, Fusarium oxysporum, and Trichoderma asperellum had the highest As-tolerance ability, based on the morphological identification and phylogenetic analysis. Culture experiment showed that on the solid plates with 30,000, 30,000, and 20,000 mg x L(-1) of As, P. janthinellum, F. oxysporum, and T. asperellum had a better colony growth, and after cultured in the liquid medium with 0-50, 0-50, and 0-80 mg x L(-1) of As for 2 days, respectively, the dry mycelia masses of the three strains all increased with increasing As concentration. When the As concentration reached 50, 50, and 80 mg x L(-1), respectively, the fungal biomass of F. oxysporum, T. asperellum, and P. janthinellum increased significantly, compared with CK. High concentration As had no significant effects on the sporalation of the three fungal strains.
从湖南省石门县和郴州市矿区采集的6份砷污染土壤样品中,共分离出13株具有较高耐砷能力的真菌菌株。根据形态学鉴定和系统发育分析,在分离出的菌株中,浅黄青霉、尖孢镰刀菌和曲霉木霉具有最高的耐砷能力。培养实验表明,在含有30000、30000和20000 mg·L⁻¹砷的固体平板上,浅黄青霉、尖孢镰刀菌和曲霉木霉的菌落生长较好,并且分别在含有0 - 50、0 - 50和0 - 80 mg·L⁻¹砷的液体培养基中培养2天后,这三种菌株的干菌丝体质量均随砷浓度的增加而增加。当砷浓度分别达到50、50和80 mg·L⁻¹时,与对照相比,尖孢镰刀菌、曲霉木霉和浅黄青霉的真菌生物量显著增加。高浓度砷对这三种真菌菌株的产孢没有显著影响。