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[水体粪便污染的微生物溯源:综述]

[Microbial source tracking of water fecal pollution: a review].

作者信息

Feng Guan-da, Deng Ming-rong, Zhu Hong-hui, Guo Jun, Zhang Xi, Zhu Chang-xiong, Liang Hao-liang

机构信息

Guangdong Province Key Laboratory of Micirobial Culture Collection and Application/Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2010 Dec;21(12):3273-81.

Abstract

Livestock feces and domestic sewage are the one of the main factors inducing water pollution, while the identification of the pollution source is particularly important in pollution control and management. Because of this, microbial source tracking (MST) has recently been paid more and more attention by the related researchers around the world. In this paper, the research progress of two types of MST methods, their advantages and disadvantages, and existing problems in application were reviewed and discussed. It was considered that in the library- and culture-dependent MST methods, PCR genotyping based on repetitive sequences was most practicable, while in the library- and culture-independent MST methods, PCR-DGGE based on the gene of specificity in Escherichia coli had a very glaring sight. Future researches should be more focused on the library- and culture-independent MST, and the combination of library- and culture-dependent MST with library- and culture-independent MST could make the tracking results more credible.

摘要

畜禽粪便和生活污水是导致水污染的主要因素之一,而污染源的识别在污染控制和管理中尤为重要。因此,微生物源追踪(MST)最近受到了全球相关研究人员越来越多的关注。本文综述并讨论了两类MST方法的研究进展、优缺点及应用中存在的问题。认为在依赖文库和培养的MST方法中,基于重复序列的PCR基因分型最具实用性,而在不依赖文库和培养的MST方法中,基于大肠杆菌特异性基因的PCR-DGGE则非常突出。未来的研究应更多地聚焦于不依赖文库和培养的MST,且将依赖文库和培养的MST与不依赖文库和培养的MST相结合可使追踪结果更可靠。

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