基于 LC-MS 的 tRNA 中 2'-核糖核苷 Ar(p)和 Gr(p)的定量分析。

LC-MS based quantification of 2'-ribosylated nucleosides Ar(p) and Gr(p) in tRNA.

机构信息

Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, LMU Munich, Butenandtstrasse 5-13, 81377 Munich, Germany.

出版信息

Chem Commun (Camb). 2011 May 14;47(18):5196-8. doi: 10.1039/c1cc11011j. Epub 2011 Mar 29.

DOI:10.1039/c1cc11011j

PMID:21448475

Abstract

RNA nucleosides are often naturally modified into complex non-canonical structures with key biological functions. Here we report LC-MS quantification of the Ar(p) and Gr(p) 2'-ribosylated nucleosides in tRNA using deuterium labelled standards, and the first detection of Gr(p) in complex fungi.

摘要

RNA 核苷通常会自然修饰成具有关键生物学功能的复杂非规范结构。在这里，我们报告了使用氘标记标准品对 tRNA 中 Ar(p) 和 Gr(p) 2'-核糖核苷进行 LC-MS 定量分析的结果，并首次在复杂真菌中检测到了 Gr(p)。