Department of Chemistry, KAIST, Daejeon 305-701, Korea.
ACS Nano. 2011 May 24;5(5):3568-76. doi: 10.1021/nn103130q. Epub 2011 Apr 6.
Among various nanoparticles, the silica nanoparticle (SiNP) is an attractive candidate as a gene delivery carrier due to advantages such as availability in porous forms for encapsulation of drugs and genes, large surface area to load biomacromolecules, biocompatibility, storage stability, and easy preparation in large quantity with low cost. Here, we report on a facile synthesis of monodispersed mesoporous silica nanoparticles (MMSN) possessing very large pores (>15 nm) and application of the nanoparticles to plasmid DNA delivery to human cells. The aminated MMSN with large pores provided a higher loading capacity for plasmids than those with small pores (∼2 nm), and the complex of MMSN with plasmid DNA readily entered into cells without supplementary polymers such as cationic dendrimers. Furthermore, MMSN with large pores could efficiently protect plasmids from nuclease-mediated degradation and showed much higher transfection efficiency of the plasmids encoding luciferase and green fluorescent protein (pLuc, pGFP) compared to MMSN with small pores (∼2 nm).
在各种纳米粒子中，由于具有多孔形式可用于封装药物和基因、具有较大的表面积可负载生物大分子、具有生物相容性、储存稳定性以及易于大量低成本制备等优点，硅纳米粒子（SiNP）是一种有吸引力的基因传递载体候选物。在这里，我们报告了一种简便的单分散介孔硅纳米粒子（MMSN）的合成方法，该纳米粒子具有非常大的孔（>15nm），并将其应用于质粒 DNA 向人细胞的传递。具有大孔的氨基化 MMSN 比具有小孔（约 2nm）的 MMSN 具有更高的质粒负载能力，并且 MMSN 与质粒 DNA 的复合物无需补充阳离子树状聚合物等即可轻易进入细胞。此外，具有大孔的 MMSN 可有效地保护质粒免受核酸酶介导的降解，并且与具有小孔（约 2nm）的 MMSN 相比，其对编码荧光素酶和绿色荧光蛋白（pLuc、pGFP）的质粒的转染效率要高得多。
