Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, Japan.
Cell Mol Life Sci. 2011 Jun;68(12):2039-51. doi: 10.1007/s00018-011-0674-x. Epub 2011 Apr 3.
Transcription is one of the most fundamental nuclear functions and is an enzyme complex-mediated reaction that converts DNA sequences into mRNA. Analyzing DNA sequences of 5'-flanking regions of several human genes that respond to 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in HL-60 cells, we have identified that the ets (GGAA) motifs are duplicated, overlapped, or clustered within a 500-bp distance from the most 5'-upstream region of the cDNA. Multiple protein factors including Ets family proteins are known to recognize and bind to the GGAA containing sequences. In addition, it has been reported that the ets motifs play important roles in regulation of various promoters. Here, we propose a molecular mechanism, defined by the presence of duplication and multiplication of the GGAA motifs, that is responsible for the initiation of transcription of several genes and for the recruitment of binding proteins to the transcription start site (TSS) of TATA-less promoters.
转录是最基本的核功能之一，是一种酶复合物介导的反应，可将 DNA 序列转换为 mRNA。我们分析了 HL-60 细胞中对 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）有反应的几种人类基因 5'-侧翼区的 DNA 序列，发现 ets（GGAA）基序在 cDNA 最上游的 500bp 距离内重复、重叠或聚集。已知包括 Ets 家族蛋白在内的多种蛋白因子可识别并结合到含有 GGAA 的序列上。此外，据报道，ets 基序在调节各种启动子方面起着重要作用。在这里，我们提出了一种分子机制，该机制由 GGAA 基序的重复和倍增所定义，负责几个基因的转录起始，并募集结合蛋白到 TATA 缺失启动子的转录起始位点（TSS）。
