克仑特罗的肌肉肥大效应与肌肉生长抑制素抑制的肥大效应具有相加作用。
The muscle-hypertrophic effect of clenbuterol is additive to the hypertrophic effect of myostatin suppression.
机构信息
Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii, USA.
出版信息
Muscle Nerve. 2011 May;43(5):700-7. doi: 10.1002/mus.21950. Epub 2011 Apr 1.
INTRODUCTION
In this study we investigated the combined effect of myostatin (MSTN) suppression and β-agonist (clenbuterol) administration on muscle hypertrophy and the phosphorylation of muscle 4E-BP1 and p70S6k, two downstream effectors of the Akt/mTOR anabolic pathway.
METHODS
Female heterozygous MSTN-prodomain transgenic mice (an MSTN suppression model) and wild-type littermates were given 0 or 20 ppm of clenbuterol (CL) in their drinking water, and muscle samples were collected at 1 and 2 weeks after treatment.
RESULTS
CL increased body and muscle mass in both genotypes. Levels of phosphorylated muscle 4E-BP1 and p70S6k were higher in MSTN-prodomain transgenic mice than in wild-type mice. CL increased the phosphorylation of 4E-BP1 and p70S6k in both genotypes.
CONCLUSIONS
The muscle-hypertrophic effect of CL is additive to the effect of MSTN suppression. The combination of MSTN suppression and treatment with β-agonists may be an effective therapeutic approach to combat muscle-wasting conditions.
简介
在这项研究中，我们研究了抑制肌肉生长抑制素（MSTN）和β-激动剂（克仑特罗）给药对肌肉肥大以及 Akt/mTOR 合成途径的两个下游效应物 4E-BP1 和 p70S6k 的磷酸化的联合影响。
方法
雌性杂合 MSTN-前导肽转基因小鼠（MSTN 抑制模型）和野生型同窝仔鼠分别给予 0 或 20ppm 的克仑特罗（CL）在饮用水中，并在治疗后 1 周和 2 周收集肌肉样本。
结果
CL 增加了两种基因型的体重和肌肉量。磷酸化肌肉 4E-BP1 和 p70S6k 的水平在 MSTN-前导肽转基因小鼠中高于野生型小鼠。CL 增加了两种基因型中 4E-BP1 和 p70S6k 的磷酸化。
结论
CL 的肌肉肥大效应是对 MSTN 抑制效应的附加作用。抑制 MSTN 与β-激动剂治疗的结合可能是对抗肌肉消耗疾病的有效治疗方法。
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