National Centre of Excellence in Molecular Biology, Lahore, Pakistan.
J Zhejiang Univ Sci B. 2011 Apr;12(4):326-34. doi: 10.1631/jzus.B1000168.
The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens. Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence. Messenger RNA (mRNA) expression in one of the transgenic lines, QCC11, was much higher than those of control and other transgenic lines. Transgenic cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance. The increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate. Data recorded up to two generations, both in the greenhouse and in the field, revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth, with 35% more yield. Moreover, the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism, decrease in apical dominance, and increase in boll size.
拟南芥的光敏色素 B（PHYB）基因通过根瘤农杆菌（Agrobacterium tumefaciens）导入棉花中。通过分子证据证实了 PHYB 基因在棉花植株中的整合和表达。在一个转基因系 QCC11 中，信使 RNA（mRNA）的表达水平远高于对照和其他转基因系。转基因棉花的光合速率提高了两倍以上，蒸腾速率和气孔导度提高了四倍以上。光合速率的提高导致相对生长率提高了 46%，净同化率提高了 18%。在温室和田间记录的两代数据表明，拟南芥 PHYB 基因在转基因棉花中的过表达通过改善棉花植株的生长，使棉花产量增加了 35%。此外，拟南芥 PHYB 基因的存在还导致了半矮化、顶端优势减弱和铃重增加等多效性效应。
