Usein Codruţa-Romaniţa, Străuţ Monica, Palade Andi-Marian, Tatu-Chiţoiu Dorina, Ciontea Simona Adriana, Băltoiu Mădălina Cornelia, Dinu Sorin, Damian Maria
Laboratory of Molecular Epidemiology, National Institute of Research-Development for Microbiology and Immunology "Cantacuzino", Bucharest, Romania.
Roum Arch Microbiol Immunol. 2010 Oct-Dec;69(4):197-203.
In Romania, Salmonella enterica serovar Typhimurium isolates are currently typed by antimicrobial resistance profiles and phage typing, as part of the national laboratory-based surveillance system of human enteric infections. The aim of the present study was to assess the added value of complementing this approach with molecular fingerprinting, namely pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem-repeats analysis (MLVA). Thirty-six S. Typhimurium isolates received by the Reference Center for Human Salmonella Infections for confirmation and typing from the Microbiology Departments of three Public Health Authorities, were selected for this study. Phage typing revealed that 14 isolates (39%) were nontypeable (NT). Twenty-two isolates were assigned to 5 phage types: DT193 (11 isolates), U302 (7 isolates), DT116 (2 isolates), DT41 (1 isolate) and DT86 (1 isolate). Antimicrobial susceptibility testing showed that all the NT and DT116 isolates were multidrug resistant and extended-spectrum betalactamase producers. All the examined isolates were typeable when using the molecular approach. Both methods gave conclusive and comparable results, documenting the genetic relatedness and discriminating the outbreak isolates from sporadic cases. We conclude that in order to improve outbreak investigation and surveillance of salmonellosis in Romania, the current routine typing of Salmonella isolates should be complemented with at least one of these DNA fingerprinting methods.
在罗马尼亚，肠炎沙门氏菌鼠伤寒血清型分离株目前通过抗菌药物耐药谱和噬菌体分型进行分型，作为国家基于实验室的人类肠道感染监测系统的一部分。本研究的目的是评估用分子指纹技术（即脉冲场凝胶电泳（PFGE）和多位点可变数目串联重复序列分析（MLVA））补充这种方法的附加价值。从三个公共卫生当局的微生物学部门接收至人类沙门氏菌感染参考中心进行确认和分型的36株鼠伤寒沙门氏菌分离株被选入本研究。噬菌体分型显示，14株分离株（39%）不可分型（NT）。22株分离株被归为5种噬菌体类型：DT193（11株）、U302（7株）、DT116（2株）、DT41（1株）和DT86（1株）。抗菌药物敏感性测试表明，所有NT和DT116分离株均为多重耐药且产超广谱β-内酰胺酶。使用分子方法时，所有检测的分离株均可分型。两种方法均给出了结论性且可比的结果，记录了遗传相关性并区分了暴发分离株和散发病例。我们得出结论，为了改进罗马尼亚沙门氏菌病的暴发调查和监测，当前沙门氏菌分离株的常规分型应至少用这些DNA指纹技术之一进行补充。
