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在成年大鼠体感皮层的多种 GABA 能神经元亚型中表达缝隙连接蛋白 connexin36。

Expression of gap junction protein connexin36 in multiple subtypes of GABAergic neurons in adult rat somatosensory cortex.

机构信息

Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.

出版信息

Cereb Cortex. 2011 Nov;21(11):2639-49. doi: 10.1093/cercor/bhr051. Epub 2011 Apr 5.

Abstract

To characterize connexin36 (Cx36)-expressing neurons of the adult rat somatosensory cortex, we examined fluorescence signals for Cx36 messenger RNA (mRNA) in 3 nonoverlapping subpopulations of γ-aminobutyric acid (GABA)ergic interneurons, which showed immunoreactivity for 1) parvalbumin (PV); 2) somatostatin (SOM); and 3) either calretinin (CR), vasoactive intestinal polypeptide (VIP), cholecystokinin (CCK), or choline acetyltransferase (ChAT). About 80% of PV-, 52% of SOM-, 37% of CR/VIP/CCK/ChAT-immunoreactive cells displayed Cx36 signals across all cortical layers, and inversely 64%, 25%, and 9% of Cx36-expressing neurons were positive for PV, SOM, or CR/VIP/CCK/ChAT, respectively. Notably, although almost all Cx36-expressing neurons in layer (L) 4, L5, and L6 were positive for one of these markers, a substantial proportion of those in L1 (91%) and L2/3 (10%) were negative for the markers tested, suggesting that other types of neurons might express Cx36. We further investigated the colocalization of Cx36 mRNA and α-actinin2 immunoreactivity, as a marker for late-spiking GABAergic neurons, by using mirror-image sections. Surprisingly, more than 77% of α-actinin2-positive cells displayed Cx36 signals in L1-L3, and about 49% and 13% of Cx36-expressing neurons were positive for α-actinin2 in L1 and L2/3, respectively. These findings suggest that all the subtypes of GABAergic interneurons might form gap junctions in the neocortex.

摘要

为了描述成年大鼠体感皮层中连接蛋白 36(Cx36)表达神经元的特征,我们在 3 种非重叠的γ-氨基丁酸(GABA)能中间神经元亚群中检测了 Cx36 信使 RNA(mRNA)的荧光信号,这些中间神经元对 1)parvalbumin(PV);2)somatostatin(SOM);和 3)calretinin(CR)、vasoactive intestinal polypeptide(VIP)、cholecystokinin(CCK)或choline acetyltransferase(ChAT)中的一种有免疫反应性。大约 80%的 PV-、52%的 SOM-、37%的 CR/VIP/CCK/ChAT-免疫反应性细胞在所有皮层层中都显示出 Cx36 信号,相反,64%、25%和 9%的 Cx36 表达神经元分别对 PV、SOM 或 CR/VIP/CCK/ChAT 呈阳性。值得注意的是,尽管 L4、L5 和 L6 层中的几乎所有 Cx36 表达神经元都对这些标志物中的一种呈阳性,但 L1(91%)和 L2/3(10%)中的相当一部分神经元对测试的标志物呈阴性,这表明可能有其他类型的神经元表达 Cx36。我们进一步通过镜像切片研究了 Cx36 mRNA 和 α-actinin2 免疫反应性的共定位,α-actinin2 作为迟发性 GABA 能神经元的标志物。令人惊讶的是,超过 77%的α-actinin2 阳性细胞在 L1-L3 中显示 Cx36 信号,大约 49%和 13%的 Cx36 表达神经元在 L1 和 L2/3 中对α-actinin2 呈阳性。这些发现表明,所有 GABA 能中间神经元亚型都可能在新皮层中形成缝隙连接。

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