Department of Medical Oncology, Medical Sciences Division, The University of Oxford, Oxford, UK.
Protein Cell. 2011 Mar;2(3):237-49. doi: 10.1007/s13238-011-1028-z. Epub 2011 Apr 6.
Rap1A is a small G protein implicated in a spectrum of biological processes such as cell proliferation, adhesion, differentiation, and embryogenesis. The downstream effectors through which Rap1A mediates its diverse effects are largely unknown. Here we show that Rap1A, but not the related small G proteins Rap2 or Ras, binds the tumor suppressor Ras association domain family 1A (RASSF1A) in a manner that is regulated by phosphorylation of RASSF1A. Interaction with Rap1A is shown to influence the effect of RASSF1A on microtubule behavior.
Rap1A 是一种小分子 G 蛋白,涉及多种生物学过程,如细胞增殖、黏附、分化和胚胎发生。Rap1A 介导其多种效应的下游效应子在很大程度上尚不清楚。本文作者显示,Rap1A(而非相关的小分子 G 蛋白 Rap2 或 Ras)以受 RASSF1A 磷酸化调节的方式与肿瘤抑制因子 Ras 相关结构域家族 1A(RASSF1A)结合。研究表明,与 Rap1A 的相互作用影响 RASSF1A 对微管行为的影响。