Department of Pathology, Medical University of South Carolina, Charleston, South Carolina, USA.
Cancer Invest. 2011 May;29(4):300-7. doi: 10.3109/07357907.2011.568564.
We screened the whole tumor genome to identify DNA copy number gains and losses that discriminate between primary breast carcinomas (MP) and their nodal metastases (ML). Six candidate genes were confirmed by quantitative PCR to have differentially distributed copy number changes. Three of the genes (ERRγ, DDX6, and TIAM1) were more commonly amplified in nodal metastases. Principal component analysis revealed that MP-ML pairs varied markedly in their genomic divergence. The latter was larger in PR-negative tumors. Nodal metastases may form early or late in the development of breast carcinomas and PR-negative tumors may metastasize earlier or are genomically less stable.
我们对整个肿瘤基因组进行了筛查，以鉴定区分原发性乳腺癌 (MP) 和其淋巴结转移 (ML) 的 DNA 拷贝数增益和缺失。通过定量 PCR 验证了六个候选基因的拷贝数变化存在差异分布。其中三个基因（ERRγ、DDX6 和 TIAM1）在淋巴结转移中更常扩增。主成分分析显示，MP-ML 对在基因组差异上有显著差异。PR 阴性肿瘤的后者差异更大。淋巴结转移可能在乳腺癌的早期或晚期形成，PR 阴性肿瘤可能更早转移或基因组更不稳定。
