原位合成标记α-甲基-5-羟色胺显示的 5-羟色胺能投射的密度：放射自显影评估。

Densities of serotonergic projections as revealed by in situ synthesised labelled α-methyl-serotonin: an autoradiographic evaluation.

机构信息

Cone Laboratory For Neurosurgical Research, Montreal Neurological Institute, and Department of Neurology and Neurosurgery, McGill University, 3801 University Street, Montreal, QC, H3A 2B4, Canada.

出版信息

Neurochem Res. 2011 Aug;36(8):1353-9. doi: 10.1007/s11064-011-0454-x. Epub 2011 Apr 7.

DOI:10.1007/s11064-011-0454-x

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3641043/

Abstract

An estimate of serotonergic innervation density and regional serotonin (5-HT) concentration was performed from the distribution of in situ produced labelled α-methyl-serotonin. Rats were injected with ((3)H) labelled α-methyl-L: -tryptophan and the tracer distribution was measured using the autoradiographic method 14 days following the injection. In a separate experiment, the total brain concentration of 5-HT in the rat brain was found to be 2.4 ± 0.2 nmol/g. Based on this, and the assumption that the specific activity of in situ produced α-methyl-serotonin is the same as that of the injected tracer, it was possible to estimate the regional concentrations of 5-HT and the relative concentration of regional serotonergic innervations. It was found, and reported for the first time here, that the highest concentration of serotonergic innervation is present in the solitary nucleus. Regionally measured 5-HT concentrations accord well with previously reported concentrations of 5-HT.

摘要

通过原位产生的标记α-甲基-5-羟色胺的分布，对 5-羟色胺能神经支配密度和 5-羟色胺（5-HT）的区域浓度进行了估计。大鼠接受标记有[（3）H]的α-甲基-L：-色氨酸注射，注射后 14 天，使用放射自显影法测量示踪剂的分布。在另一个实验中，发现大鼠脑中 5-HT 的总脑浓度为 2.4±0.2 nmol/g。基于此，并假设原位产生的α-甲基-5-羟色胺的比活度与注入的示踪剂相同，因此可以估计 5-HT 的区域浓度和区域 5-羟色胺能神经支配的相对浓度。结果发现，这里首次报道，孤束核存在最高密度的 5-羟色胺能神经支配。区域性测量的 5-HT 浓度与先前报道的 5-HT 浓度一致。

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