Diagnosis of tubercular uveitis by quantitative polymerase chain reaction.
作者信息
Sharma Pooja, Bansal Reema, Gupta Vishali, Gupta Amod
机构信息
Department of Ophthalmology, Advanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012 India.
出版信息
J Ophthalmic Inflamm Infect. 2010 Nov 11;1(1):23-7. doi: 10.1007/s12348-010-0004-8.
PURPOSE
To report the use of real-time or quantitative polymerase chain reaction (qPCR) in confirming the diagnosis of tubercular uveitis.
METHODS
A qPCR assay using primers targeting the MPB64 gene of Mycobacterium tuberculosis (MTB) was developed. A standard curve for quantification of mycobacterial load was generated. Vitreous fluid samples from patients presumed to have tubercular uveitis were assayed to identify and quantify the mycobacterial load. The amount of the test sample product was interpolated from the standard curve of cycle threshold values generated from known starting copy number of standard MTB DNA.
RESULTS
The copies of MTB genomes in the three test samples studied were found to be 7.61 × 10(4), 4.53 × 10(4), and 1.43 × 10(5), respectively.
CONCLUSION
qPCR detected and quantified MTB genome from the vitreous fluid of eyes clinically suspected with tubercular uveitis.
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