State University of New York College of Environmental Science and Forestry, Department of Environmental and Forest Biology, 1 Forestry Drive, Syracuse, New York 13210, USA.
Mycologia. 2011 Jul-Aug;103(4):722-30. doi: 10.3852/10-334. Epub 2011 Apr 11.
Several recent fine-scale genetic structure studies of ectomycorrhizal fungi have reported significant spatial clustering of genets with similar genotypes, supporting locally restricted gene flow. In this study we used genotype data from microsatellite markers and spatial autocorrelation analysis to examine local gene flow in Suillus spraguei at distances up to 2 km. Previously developed microsatellite markers for S. spraguei from Japan were unsuccessful at amplifying DNA isolated from sporocarps found in New York state, and other research suggested that both are disjunct species. Novel microsatellite markers therefore were developed with New York specimens. We identified nine polymorphic microsatellite loci and developed primer sets to amplify these regions. We tested the efficiency of the primer sets on 50 sporocarps collected from a natural Pinus strobus stand. The majority of the markers were in Hardy-Weinberg and linkage equilibrium. The location of all sampled sporocarps was recorded and used along with multilocus genotype data to create a genet map. The distance between sporocarps with the same multilocus genotype was small (≤ 7.65 m) and the majority of sporocarps collected were genetically unique, suggesting frequent spore establishment and sexual recombination on this site. Spatial autocorrelation analysis did not support clustering of similar genotypes, suggesting few restrictions to gene flow within this local population.
最近有几项关于外生菌根真菌的精细遗传结构研究报告称，具有相似基因型的基因在空间上存在显著的聚类，支持局部限制基因流。在这项研究中，我们使用来自微卫星标记的基因型数据和空间自相关分析，在长达 2 公里的范围内研究了苏氨酸 spraguei 的局部基因流。先前从日本开发的用于 S. spraguei 的微卫星标记在扩增从纽约州发现的子实体中分离的 DNA 时不成功，其他研究表明两者都是间断种。因此，我们使用纽约州的标本开发了新的微卫星标记。我们鉴定了九个多态性微卫星位点，并开发了引物对来扩增这些区域。我们在从天然的火炬松林中采集的 50 个子实体上测试了引物对的效率。大多数标记处于哈迪-温伯格和连锁平衡状态。所有采样子实体的位置都被记录下来，并与多位点基因型数据一起用于创建基因图谱。具有相同多位点基因型的子实体之间的距离很小（≤7.65m），并且采集到的大多数子实体在遗传上都是独特的，这表明在该地点经常发生孢子建立和有性重组。空间自相关分析不支持相似基因型的聚类，这表明在该局部种群中基因流很少受到限制。
