Department of Neurosurgery and Division of Pediatric Neurosurgery, University of Utah, Salt Lake City, Utah 84132, USA.
J Biomed Mater Res A. 2011 Jun 15;97(4):433-40. doi: 10.1002/jbm.a.33078. Epub 2011 Apr 11.
While silicone devices have vastly improved an array of medical treatments, reactions at the tissue-substrate interface often impede their functionality. Insertion of a poly(dimethyl)siloxane (PDMS) catheter into the cerebral ventricles to drain excess cerebrospinal fluid (CSF) is the most common treatment of hydrocephalus, but shunting often fails because inflammatory tissue, choroid plexus cells, and debris grow into these central nervous system catheters and obstruct flow. We hypothesized that plasma oxidation of PDMS would inhibit macrophage and astrocyte adhesion under flow (0 to 0.3 mL/min) and protein (20.8 to 240 mg/dL) conditions similar to those observed in the physiological state. Oxidation (to increase wettability) had an inhibitory effect on macrophage cell binding (yielding a significant 88% change) that was generally more pronounced than the effect of flow (22% change) or protein concentration (3% change). In contrast, greater flow increased binding of astrocytes in most cases (yielding a significant 97% change); plasma oxidation (19% change), and protein concentration (60% change) had less pronounced effects. This study is the initial indicator that plasma oxidation of PDMS catheters may inhibit macrophage adhesion during CSF outflow but may not be as effective at inhibiting astrocyte binding.
虽然硅树脂装置极大地改善了一系列医学治疗方法，但在组织-基底界面的反应常常会妨碍其功能。将聚二甲基硅氧烷（PDMS）导管插入脑室内以排出过多的脑脊液（CSF）是治疗脑积水最常见的方法，但分流常常失败，因为炎症组织、脉络丛细胞和碎片会生长到这些中枢神经系统导管中并阻碍流动。我们假设 PDMS 的等离子体氧化在类似于生理状态下观察到的流动（0 到 0.3 mL/min）和蛋白质（20.8 到 240 mg/dL）条件下会抑制巨噬细胞和星形胶质细胞的黏附。氧化（增加润湿性）对巨噬细胞黏附具有抑制作用（产生显著的 88%变化），这通常比流动（22%变化）或蛋白质浓度（3%变化）的影响更为明显。相比之下，在大多数情况下，更大的流动会增加星形胶质细胞的黏附（产生显著的 97%变化）；等离子体氧化（19%变化）和蛋白质浓度（60%变化）的影响则不那么明显。本研究初步表明，PDMS 导管的等离子体氧化可能在 CSF 流出过程中抑制巨噬细胞黏附，但在抑制星形胶质细胞结合方面可能效果不佳。
