Hager K M, Yanofsky C
Department of Biological Sciences, Stanford University, CA 94305.
Gene. 1990 Dec 15;96(2):153-9. doi: 10.1016/0378-1119(90)90247-o.
Asexual development in Neurospora crassa proceeds through a series of discrete morphological stages that culminate in the production of dormant spores called conidia. Changes in the pattern of gene expression parallel the morphological transformations associated with conidiation. As a prerequisite to the analysis of developmental gene expression in N. crassa, several genes of unknown function that are preferentially expressed during conidiation were isolated [Berlin and Yanofsky, Mol. Cell. Biol. 5 (1985) 849-855]. The molecular structure and nucleotide sequence of one of these genes, designated con-13, is presented. The con-13 gene specifies a relatively rare 1.35-kb message which is first detected about 8 h following the induction of conidiation. Sequence analysis of both cDNA and genomic clones indicates that the con-13 gene consists of three exons divided by two small introns. It encodes a polypeptide of 340 amino acid residues (37.1 kDa). The Con-13 protein is weakly acidic and hydrophilic. A comparison of the regions upstream from the con-8, con-10, and con-13 genes revealed several short sequence motifs which may be important in developmental gene regulation.
粗糙脉孢菌的无性发育通过一系列离散的形态学阶段进行，最终产生称为分生孢子的休眠孢子。基因表达模式的变化与分生孢子形成相关的形态转变平行。作为分析粗糙脉孢菌发育基因表达的前提条件，分离出了几个在分生孢子形成过程中优先表达的功能未知的基因[柏林和亚诺夫斯基，《分子细胞生物学》5(1985)849 - 855]。本文介绍了其中一个名为con - 13的基因的分子结构和核苷酸序列。con - 13基因指定了一种相对罕见的1.35 kb的信息，在分生孢子形成诱导后约8小时首次检测到。cDNA和基因组克隆的序列分析表明，con - 13基因由三个外显子组成，被两个小内含子隔开。它编码一个由340个氨基酸残基组成的多肽(37.1 kDa)。Con - 13蛋白呈弱酸性且亲水。对con - 8、con - 10和con - 13基因上游区域的比较揭示了几个短序列基序，这些基序可能在发育基因调控中很重要。
