Cátedra de Química Biológica, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, UBA, Buenos Aires, Argentina.
Andrologia. 2011 Dec;43(6):409-15. doi: 10.1111/j.1439-0272.2010.01094.x. Epub 2011 Apr 6.
Boar spermatozoa are sensitive to oxidative damage produced during cryopreservation. Our aim was to evaluate the participation of different antioxidants in the improvement of cryopreserved boar sperm functionality. Spermatozoa frozen with 200 μg ml(-1) α-tocopherol, 0.5 mm 17β-oestradiol or seminal plasma were used to evaluate sperm parameters and capacitation-like changes. The 17β-oestradiol and α-tocopherol concentrations were assessed by RIA and HPLC respectively. Motility was improved but lipid peroxidation and capacitation-like changes were diminished (P < 0.05) in antioxidant samples. A significant increase in 17β-oestradiol concentration was detected in 17β-oestradiol or seminal plasma samples. Alpha-tocopherol content increased in α-tocopherol, 17β-oestradiol or seminal plasma samples, obtaining the lowest level in the α-tocopherol ones. The 17β-oestradiol or seminal plasma components may be acting in the regeneration of the α-tocopherol antioxidant capacity. The α-tocopherol concentration may be conditioning the cryopreserved boar sperm functionality. The addition of antioxidants could be useful to reduce oxidative stress, thus improving the functionality of cryopreserved boar spermatozoa.
猪精子对冷冻过程中产生的氧化损伤敏感。我们的目的是评估不同抗氧化剂在改善冷冻猪精子功能中的作用。用 200μgml(-1)α-生育酚、0.5mm 17β-雌二醇或精液对精子进行冷冻，以评估精子参数和类似获能的变化。通过 RIA 和 HPLC 分别评估 17β-雌二醇和α-生育酚的浓度。抗氧化剂样品中精子的活力提高，但脂质过氧化和类似获能的变化减少（P < 0.05）。在 17β-雌二醇或精液样品中检测到 17β-雌二醇浓度显著增加。α-生育酚含量在α-生育酚、17β-雌二醇或精液样品中增加，在α-生育酚样品中含量最低。17β-雌二醇或精液成分可能在α-生育酚抗氧化能力的再生中起作用。α-生育酚浓度可能影响冷冻猪精子的功能。添加抗氧化剂可以减少氧化应激，从而改善冷冻猪精子的功能。
