Institute for Veterinary Biochemistry and Molecular Biology, University of Zürich-Irchel, Winterthurerstrasse 190, 8057 Zürich, Switzerland.
FEBS Lett. 2011 Sep 16;585(18):2826-30. doi: 10.1016/j.febslet.2011.03.069. Epub 2011 Apr 8.
DNA polymerase (pol) λ, one of the 15 cellular pols, belongs to the X family. It is a small 575 amino-acid protein containing a polymerase, a dRP-lyase, a proline/serine rich and a BRCT domain. Pol λ shows various enzymatic activities including DNA polymerization, terminal transferase and dRP-lyase. It has been implicated to play a role in several DNA repair pathways, particularly base excision repair (BER), non-homologous end-joining (NHEJ) and translesion DNA synthesis (TLS). Similarly to other DNA repair enzymes, pol λ undergoes posttranslational modifications during the cell cycle that regulate its stability and possibly its subcellular localization. Here we describe our knowledge about ubiquitylation of pol λ and the impact of this modification on its regulation.
DNA 聚合酶（pol）λ是 15 种细胞 pol 之一，属于 X 家族。它是一种含有聚合酶、dRP 内切酶、脯氨酸/丝氨酸丰富区和 BRCT 结构域的 575 个氨基酸的小蛋白。Pol λ 显示出多种酶活性，包括 DNA 聚合、末端转移酶和 dRP 内切酶。它被认为在几种 DNA 修复途径中发挥作用，特别是碱基切除修复（BER）、非同源末端连接（NHEJ）和跨损伤 DNA 合成（TLS）。与其他 DNA 修复酶一样，pol λ 在细胞周期中经历翻译后修饰，调节其稳定性和可能的亚细胞定位。在这里，我们描述了我们对 pol λ 泛素化的认识，以及这种修饰对其调控的影响。
