一种用于蛋白质构象和氧化还原状态的小型荧光报告基团。

A small fluorophore reporter of protein conformation and redox state.

机构信息

Department of Chemistry, Dartmouth College, Hanover, NH 03755, USA.

出版信息

Chem Commun (Camb). 2011 May 28;47(20):5714-6. doi: 10.1039/c1cc10896d. Epub 2011 Apr 12.

Abstract

A new thiol-specific reagent introduces a small bis(methylamino)terephthalic acid fluorophore into proteins. The noninvasive probe with distinct spectroscopic properties offers many advantages for protein labeling, purification, and mechanistic work promising to serve as a powerful tool in studies of protein folding and heme redox reactions.

摘要

一种新的巯基特异性试剂将一个小的双（甲基氨基）对苯二甲酸荧光团引入蛋白质中。这种具有独特光谱特性的非侵入性探针为蛋白质标记、纯化和机制研究提供了许多优势，有望成为研究蛋白质折叠和血红素氧化还原反应的有力工具。