Laboratory of Advanced Materials, State Key Laboratory of New Ceramic & Fine Processing, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Arch Oral Biol. 2011 Oct;56(10):1020-6. doi: 10.1016/j.archoralbio.2011.03.010. Epub 2011 Apr 13.
Enamel mineralisation is a highly controlled process in which the deposition, growth, and maturation of inorganic crystallites are regulated by secreted matrix proteins at the molecular and cellular level. Maxillary and mandibular first molars from the col1-caPPR mutants as well as normal controls aged for 12 weeks were observed by SEM and nanoindentation, respectively. Several types of aberrations in enamel distribution and crystal organisation were encountered in the transgenic molars. Also, the gene alteration resulted in degradation by as much as 23.42% in hardness and 17.56% in the elastic modulus. These data suggested that gene mutation altered the ameloblastic differentiation and movement, resulting in variations of crystal arrangement patterns, aberrations of enamel distribution, and degradation of mechanical behaviour. Furthermore, the col1-caPPR mouse model was determined as useful for studying how the genes modulate the biomineralisation process.
釉质矿化是一个高度受控的过程，其中无机晶体的沉积、生长和成熟受分子和细胞水平上分泌的基质蛋白调节。用 SEM 和纳米压痕法分别观察了 col1-caPPR 突变体的上颌和下颌第一磨牙以及正常对照的 12 周龄。在转基因磨牙中遇到了几种类型的釉质分布和晶体组织异常。此外，基因改变导致硬度降低 23.42%，弹性模量降低 17.56%。这些数据表明，基因突变改变了成釉细胞的分化和运动，导致晶体排列模式的变化、釉质分布的异常和机械性能的降低。此外，col1-caPPR 小鼠模型被确定为研究基因如何调节生物矿化过程的有用模型。
