Dai Yan-cheng, Tang Zhi-peng, Wang Zhen-nan, Zhang Ya-li, He Xin-ying
Department of Gastroenterology, Longhua Hospital, Shanghai, China.
Chin Med Sci J. 2011 Mar;26(1):43-8. doi: 10.1016/s1001-9294(11)60018-7.
To observe the influence of Shenqing Recipe (SQR), a kind of Traditional Chinese Medicine, on the morphology and quantity of colonic interstitial cells of Cajal (ICC) in trinitrobenzene sulfonic acid (TNBS)-induced rat colitis, and to investigate the possible mechanism of SQR in regulating intestinal dynamics.
Sixty rats were randomly divided into normal control, model 1, model 2, mesalazine, and high-dose, and low-dose SQR groups with 10 rats in each group. TNBS (10 mg) dissolved in 50% ethanol was instilled into the lumen of the rat colon of the latter five groups to induce colitis. On the 4th day after administration of TNBS, each treatment group was administered one of the following formulations by enteroclysis gavage once a day for 7 days: 600 mg•kg⁻¹•d⁻¹ mesalazine, 2.4 g•kg⁻¹•d⁻¹ SQR, and 1.2 g•kg⁻¹•d⁻¹ SQR. Model 2 rats received normal saline solution. After 7 days colonic samples were collected. While the colonic samples of model 1 group were collected on the 3rd day after TNBS administered. Ultrastructure of ICC in the damaged colonic tissues was observed with transmission electron microscope. Expression of c-kit protein in colonic tissue was determined by immunohistochemical staining and Western blot.
The ultrastructure of colonic ICC in the rat model of TNBS-induced colitis showed a severe injury, and administration of SQR or mesalazine reduced the severity of injury. Similarly, the expression of c-kit protein of TNBS-induced colitis rat model was significantly decreased compared with the normal control group (P < 0.05). Treatment with SQR or mesalazine significantly increased the expression of c-kit protein compared with the administration of control formulations (P < 0.05), especially the high-dose SQR group.
SQR could alleviate and repair the injured ICC, and improve its quantity, which might be involved in regulating intestinal motility.
观察中药参芩方（SQR）对三硝基苯磺酸（TNBS）诱导的大鼠结肠炎结肠Cajal间质细胞（ICC）形态及数量的影响，探讨SQR调节肠道动力的可能机制。
将60只大鼠随机分为正常对照组、模型1组、模型2组、美沙拉嗪组、SQR高剂量组和SQR低剂量组，每组10只。后五组大鼠结肠内注入溶于50%乙醇的TNBS（10 mg）诱导结肠炎。TNBS给药后第4天，各治疗组每天经灌肠法灌胃给予以下制剂之一，连续7天：600 mg•kg⁻¹•d⁻¹美沙拉嗪、2.4 g•kg⁻¹•d⁻¹ SQR、1.2 g•kg⁻¹•d⁻¹ SQR。模型2组大鼠给予生理盐水。7天后采集结肠样本。模型1组结肠样本在TNBS给药后第3天采集。用透射电子显微镜观察受损结肠组织中ICC的超微结构。通过免疫组织化学染色和蛋白质印迹法检测结肠组织中c-kit蛋白的表达。
TNBS诱导的结肠炎大鼠模型结肠ICC超微结构显示严重损伤，给予SQR或美沙拉嗪可减轻损伤程度。同样，TNBS诱导的结肠炎大鼠模型c-kit蛋白表达与正常对照组相比显著降低（P < 0.05）。与给予对照制剂相比，SQR或美沙拉嗪治疗显著增加了c-kit蛋白的表达（P < 0.05），尤其是SQR高剂量组。
SQR可减轻并修复受损的ICC，增加其数量，这可能与调节肠道动力有关。
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