Department of Animal Sciences, University of Florida, Florida, USA.
Theriogenology. 2011 Sep 1;76(4):615-22. doi: 10.1016/j.theriogenology.2011.03.014. Epub 2011 Apr 16.
The objectives were to evaluate the effects of exercise on ovarian folliculogenesis and related hormones in mares. Mares (n = 11) were randomly assigned into a control (non-exercised) or treatment (exercised) group. Treatment mares (n = 5) were moderately exercised for 30 min, 6 d/wk. All mares underwent daily transrectal ultrasonographic examinations and ovarian follicles > 6 mm were measured. Blood samples were collected during the first (Cycle 1) and last (Cycle 4) cycle, and serum concentrations of cortisol, LH, and FSH were determined. Mean cortisol concentrations were elevated (P < 0.05) in exercised mares, 6.29 ± 0.22 compared with 5.62 ± 0.16 ng/dL (mean ± SEM), 30 min post exercise. There were no significant differences between groups in mean FSH concentrations; however, exercised mares had lower (17.3 ± 6.4 vs 41.1 ± 5.5 ng/mL; P < 0.05) peak LH concentrations. Furthermore, exercised mares experienced a longer (24.7 ± 0.8 vs 22.2 ± 0.8 d; P < 0.05) mean interovulatory interval for all cycles combined, fewer (P < 0.05) follicles 6 to 20 mm in diameter, and an increased (P < 0.05) number of follicles >20 mm following deviation. The dominant and largest subordinate follicle in exercised mares had a greater (P < 0.05) mean diameter on the day of deviation, suggesting delayed deviation. Exercised mares also tended (P = 0.06) to have an increased number of cycles with at least two dominant follicles compared to control (62 vs 36%, respectively), indicating a decreased ability of the largest follicle to assert dominance. Under the conditions of this study, moderately exercising mares induced higher cortisol concentrations, lowered peak LH concentrations, and altered ovarian follicular dynamics.
目的是评估运动对母马卵巢卵泡发生和相关激素的影响。将 11 匹母马随机分为对照组（未运动）和治疗组（运动）。治疗组母马（n=5）每周运动 6 天，每天运动 30 分钟。所有母马均进行每日经直肠超声检查，测量>6mm 的卵巢卵泡。在第一个（周期 1）和最后一个（周期 4）周期采集血液样本，测定血清皮质醇、LH 和 FSH 浓度。运动后 30 分钟，运动母马的皮质醇浓度升高（P<0.05），为 6.29±0.22ng/dL（均值±SEM），而对照组为 5.62±0.16ng/dL。两组 FSH 浓度均值无显著差异；然而，运动母马的 LH 峰浓度较低（17.3±6.4 比 41.1±5.5ng/mL；P<0.05）。此外，运动母马的所有周期平均排卵间隔较长（24.7±0.8 比 22.2±0.8d；P<0.05），6 至 20mm 直径的卵泡较少（P<0.05），偏离后>20mm 的卵泡增多（P<0.05）。运动母马的优势和最大的次级卵泡在偏离日的平均直径较大（P<0.05），提示偏离延迟。与对照组相比，运动母马的周期中至少有两个优势卵泡的数量也有增加的趋势（P=0.06），表明最大卵泡的优势确立能力下降。在本研究条件下，适度运动母马可诱导更高的皮质醇浓度、降低 LH 峰浓度，并改变卵巢卵泡动力学。
