真核细胞质最小化蛋白的计算机模拟和体内研究。
In silico and in vivo investigations of proteins of a minimized eukaryotic cytoplasm.
机构信息
LOEWE-Zentrum für Synthetische Mikrobiologie (SynMikro), Hans-Meerwein-Strasse, Marburg, Germany.
出版信息
Genome Biol Evol. 2011;3:375-82. doi: 10.1093/gbe/evr031. Epub 2011 Apr 17.
Abstract
Algae with secondary plastids such as diatoms maintain two different eukaryotic cytoplasms. One of them, the so-called periplastidal compartment (PPC), is the naturally minimized cytoplasm of a eukaryotic endosymbiont. In order to investigate the protein composition of the PPC of diatoms, we applied knowledge of the targeting signals of PPC-directed proteins in searches of the genome data for proteins acting in the PPC and proved their in vivo localization via expressing green fluorescent protein (GFP) fusions. Our investigation increased the knowledge of the protein content of the PPC approximately 3-fold and thereby indicated that this narrow compartment was functionally reduced to some important cellular functions with nearly no housekeeping biochemical pathways.
摘要
具有次级质体（如硅藻）的藻类维持着两种不同的真核细胞质。其中之一，所谓的周质腔（PPC），是一种真核内共生体自然最小化的细胞质。为了研究硅藻 PPC 的蛋白质组成，我们应用了 PPC 定向蛋白靶向信号的知识，在基因组数据中搜索作用于 PPC 的蛋白，并通过表达绿色荧光蛋白（GFP）融合体来证明它们的体内定位。我们的研究使 PPC 的蛋白质含量增加了约 3 倍，从而表明这个狭窄的腔室在功能上已经减少到一些重要的细胞功能，几乎没有维持生物化学途径。
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Photosynth Res. 2011 Sep;109(1-3):205-21. doi: 10.1007/s11120-011-9634-4. Epub 2011 Mar 2.
2
Inorganic carbon acquisition by eukaryotic algae: four current questions.
Photosynth Res. 2010 Nov;106(1-2):123-34. doi: 10.1007/s11120-010-9563-7. Epub 2010 Jun 4.
3
New mechanistic insights into pre-protein transport across the second outermost plastid membrane of diatoms.
Mol Microbiol. 2010 May;76(3):793-801. doi: 10.1111/j.1365-2958.2010.07142.x. Epub 2010 Mar 25.
4
Filling the gap, evolutionarily conserved Omp85 in plastids of chromalveolates.
J Biol Chem. 2010 Feb 26;285(9):6848-56. doi: 10.1074/jbc.M109.074807. Epub 2009 Dec 30.
5
The presence and localization of thioredoxins in diatoms, unicellular algae of secondary endosymbiotic origin.
Mol Plant. 2009 May;2(3):468-77. doi: 10.1093/mp/ssp010. Epub 2009 Mar 19.
6
Genetic evidence that an endosymbiont-derived endoplasmic reticulum-associated protein degradation (ERAD) system functions in import of apicoplast proteins.
J Biol Chem. 2009 Nov 27;284(48):33683-91. doi: 10.1074/jbc.M109.044024. Epub 2009 Oct 6.
7
A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal degradation.
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8
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9
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