Institute of Pathology, University Hospital Jena, Friedrich-Schiller-University Jena, Ziegelmühlenweg 1, Jena 07743, Germany.
Int J Oncol. 2011 Jul;39(1):161-8. doi: 10.3892/ijo.2011.1010. Epub 2011 Apr 18.
Human complement factor H (CFH), a central complement control protein, is a member of the regulators of complement activation family. Recent studies suggested that CFH may play a key role in the resistance of complement-mediated lysis in various cancer cells. In this study, we investigated the role of CFH in human lung cancer. Expression of CFH was analyzed in lung cancer cell lines by RT-PCR, Western blotting and immunofluorescence. In primary lung tumors, the protein expression of CFH was evaluated by immunohistochemistry (IHC) on tissue microarray (TMA). Binding of CFH to lung cancer cells was detected by flow cytometry. mRNA expression of CFH was detected in 6 out of 10 non-small cell lung cancer (NSCLC) cell lines, but in none of the small cell lung cancer (SCLC) cell lines. In line with Western blotting, immunofluorescence analysis demonstrated CFH protein expression in 3 NSCLC cell lines, and the immunoreaction was mainly associated with cell cytoplasm and membrane. In primary lung tumors, 54 out of 101 samples exhibited high expression of CFH and high expression was significantly correlated with lung adenocarcinoma (p=0.009). Also, in adenocarcinoma of the lung, Kaplan-Meier survival analysis showed a tendency that CFH-positive tumors had worse prognosis in comparison to CFH-negative tumors (p=0.082). Additionally, shorter survival time of patients with adenocarcinoma (<20 months) was associated with higher staining of CFH (p=0.033). Our data showed that non-small cell lung cancer cells expressed and secreted CFH. CFH might be a novel diagnostic marker for human lung adenocarcinoma.
人类补体因子 H（CFH）是一种重要的补体调控蛋白，属于补体激活调节剂家族成员。最近的研究表明，CFH 可能在各种癌细胞中对补体介导的溶解具有抵抗作用。在本研究中，我们研究了 CFH 在人类肺癌中的作用。通过 RT-PCR、Western blot 和免疫荧光法分析肺癌细胞系中 CFH 的表达。通过免疫组织化学（IHC）在组织微阵列（TMA）上评估原发性肺肿瘤中 CFH 的蛋白表达。通过流式细胞术检测 CFH 与肺癌细胞的结合。在 10 个非小细胞肺癌（NSCLC）细胞系中的 6 个中检测到 CFH 的 mRNA 表达，但在小细胞肺癌（SCLC）细胞系中均未检测到。与 Western blot 一致，免疫荧光分析显示 3 个 NSCLC 细胞系中存在 CFH 蛋白表达，免疫反应主要与细胞质和细胞膜相关。在原发性肺肿瘤中，101 个样本中有 54 个表现出 CFH 的高表达，高表达与肺腺癌显著相关（p=0.009）。此外，在肺腺癌中，Kaplan-Meier 生存分析表明 CFH 阳性肿瘤的预后较 CFH 阴性肿瘤差（p=0.082）。此外，腺癌患者的生存时间（<20 个月）较短与 CFH 染色强度较高相关（p=0.033）。我们的数据表明，非小细胞肺癌细胞表达和分泌 CFH。CFH 可能是人类肺腺癌的一种新的诊断标志物。
