Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
Clin Biochem. 2011 Jul;44(10-11):771-8. doi: 10.1016/j.clinbiochem.2011.04.008. Epub 2011 Apr 22.
To determine age and gender specific reference intervals for bone markers.
Morning blood samples were collected after overnight fast from 356 healthy children (6 to 18 year-old) for the determination of bone marker levels, PTH and vitamin D(3). Multiple regression analysis was done to assess the effect of factors that could influence the bone marker levels; the central 95% reference interval and their 90% CI were calculated.
After excluding samples when BMI-z -scores were <-2 or >+2, both vitamin D(3) and PTH levels were abnormal and from children who used steroids the remainder were partitioned using Tanner stage based chronological age. As expected the reference intervals show a significant variation with age and gender. All the bone marker levels, except total alkaline phosphatase, were significantly higher in puberty with vitamin D(3) >75nmol/L.
Vitamin D status, age and gender are important for establishing reference intervals of bone markers in healthy children.
确定骨标志物的年龄和性别特异性参考区间。
从 356 名健康儿童（6 至 18 岁）中采集过夜禁食后的早晨血液样本，以测定骨标志物水平、甲状旁腺激素（PTH）和维生素 D（3）。采用多元回归分析评估可能影响骨标志物水平的因素的影响；计算中心 95%参考区间及其 90%CI。
排除 BMI-z 评分<-2 或>+2 的样本后，维生素 D（3）和 PTH 水平异常，且使用类固醇的儿童的样本根据青春期的年龄进行了分组。正如预期的那样，参考区间随年龄和性别而有显著变化。除总碱性磷酸酶外，所有骨标志物水平在维生素 D（3）>75nmol/L 的青春期均显著升高。
维生素 D 状态、年龄和性别对于确定健康儿童骨标志物的参考区间非常重要。
