滤纸干血斑法检测α-半乳糖苷酶 A、芳基硫酸酯酶 B 和α-葡萄糖苷酶活性的影响因素分析。
Influence of pre-analytical factors on α-galactosidase A, arylsulfatase B and α-glucosidase activities measured on dried blood spots on filter paper.
机构信息
Department and Program of Post-graduation in Biochemistry, Federal University of Rio Grande do Sul, UFRGS, Porto Alegre, RS, Brazil.
出版信息
Clin Biochem. 2011 Jul;44(10-11):922-6. doi: 10.1016/j.clinbiochem.2011.03.138. Epub 2011 Apr 20.
OBJECTIVES
To analyze the effect of blood collection and storage conditions on activity of α-galactosidase A, arylsulfatase B and α-glucosidase.
DESIGN AND METHODS
Blood was collected in EDTA, heparin, or direct spotting on filter paper and stored at different temperatures (-20, 4, 25 and 37°C) and storage times (3, 10, 17 and 180 days). The influence of filter paper size was also assessed (3.0 and 1.2mm).
RESULTS
No statistically significant difference was observed between the three collection methods. α-Glucosidase A activity significantly decreased after the 10th day, while arylsulfatase B activity only differed significantly after the 180th day, and α-galactosidase A activity remained constant throughout this storage time. Excellent correlation coefficients were observed for the two filter paper sizes used.
CONCLUSIONS
Both paper sizes may be employed. Filter paper specimens should be transported under refrigeration as soon as possible after blood collection.
目的
分析采血和储存条件对α-半乳糖苷酶 A、芳基硫酸酯酶 B 和α-葡萄糖苷酶活性的影响。
设计和方法
用 EDTA、肝素或直接点样在滤纸上采集血液，并在不同温度（-20°C、4°C、25°C 和 37°C）和储存时间（3 天、10 天、17 天和 180 天）下储存。还评估了滤纸条大小（3.0mm 和 1.2mm）的影响。
结果
三种采集方法之间无统计学显著差异。α-葡萄糖苷酶 A 活性在第 10 天后显著降低，而芳基硫酸酯酶 B 活性仅在第 180 天后才有显著差异，α-半乳糖苷酶 A 活性在整个储存时间内保持不变。两种所用滤纸条尺寸之间观察到了极好的相关系数。
结论
两种滤纸尺寸都可以使用。滤纸条标本在采血后应尽快冷藏运输。
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