Department of Microbiology & Cell Science, University of Florida, Box 110700, Gainesville, FL 32611, USA.
Bioresour Technol. 2011 Jul;102(13):6959-65. doi: 10.1016/j.biortech.2011.04.036. Epub 2011 Apr 15.
Microaeration (injecting air into the headspace) improved the fermentation of hemicellulose hydrolysates obtained from the phosphoric acid pretreatment of sugarcane bagasse at 170°C for 10 min. In addition, with 10% slurries of phosphoric acid pretreated bagasse (180°C, 10 min), air injection into the headspace promoted xylose utilization and increased ethanol yields from 0.16 to 0.20 g ethanol/g bagasse dry weight using a liquefaction plus simultaneous saccharification and co-fermentation process (L+SScF). This process was scaled up to 80 L using slurries of acid pretreated bagasse (96 h incubation; 0.6L of air/min into the headspace) with ethanol yields of 312-347 L (82-92 gal) per tone (dry matter), corresponding to 0.25 and 0.27 g/g bagasse (dry weight). Injection of small amounts of air into the headspace may provide a convenient alternative to subsurface sparging that avoids problems of foaming, sparger hygiene, flotation of particulates, and phase separation.
微需氧（向顶部空间注入空气）可改善 170°C 下磷酸预处理甘蔗渣 10 分钟得到的半纤维素水解产物的发酵。此外，在 10%的磷酸预处理蔗渣（180°C，10 分钟）浆中，向顶部空间注入空气可促进木糖的利用，并在液化+同步糖化共发酵（L+SScF）过程中使乙醇产量从 0.16 提高到 0.20 g 乙醇/蔗渣干重。该工艺在 80L 规模下使用酸预处理蔗渣浆（96h 孵育；顶部空间每分钟 0.6L 空气）进行放大，乙醇产量为 312-347L（82-92 加仑）/吨（干物质），对应于 0.25 和 0.27g/g 蔗渣（干重）。向顶部空间注入少量空气可能是替代底层曝气的一种便捷方法，可避免起泡、曝气卫生、颗粒浮升和相分离等问题。
