根际细菌的抗真菌活性。

Antifungal activity of rhizospheric bacteria.

作者信息

Mezaache S, Guechi A, Zerroug M M, Strange R N, Nicklin J

机构信息

University of Sétif, Faculty of Sciences, Dept. Biology, Laboratory of Applied Microbiology, 19000 Sétif, Algeria.

出版信息

Commun Agric Appl Biol Sci. 2010;75(4):671-4.

PMID:21534477

Abstract

Fluorescent Pseudomonad spp. were isolated from the rhizosphere of potato plants (Algeria) by serial dilutions of rhizosphere soils on Kings B medium and were tested for their antifungal activity. The antifungal activity of the Pseudomonas isolated from Potatoes rhizosphere was tested against Pythium ultimum, Rhizoctonia solani and Fusarium oxysporum in dual culture with bacteria on PDA. The Petri dish was divided into tow, on one the bacteria was spread and on the opposite side fungal plugs were inoculated and incubated for one week. Fourteen bacteria were isolated; only one isolate inhibited the growth of Pythium ultimum, Rhizoctonia solani, Fusarium solani; Fusarium oxysporum f.sp. albedinis and Fusarium oxysporum f. sp. Lycopersici with inhibition zones of 39.9, 33.7, 30.8, 19.9 and 22.5 mm respectively.

摘要

荧光假单胞菌属细菌是通过将阿尔及利亚马铃薯植株根际土壤在金氏B培养基上进行系列稀释而从根际分离得到的，并对其抗真菌活性进行了测试。从马铃薯根际分离出的假单胞菌的抗真菌活性，在PDA上与细菌进行双重培养时，针对终极腐霉、立枯丝核菌和尖孢镰刀菌进行了测试。培养皿被分成两部分，一部分涂布细菌，另一侧接种真菌菌块，并培养一周。共分离出14株细菌；只有一株分离物抑制了终极腐霉、立枯丝核菌、茄形镰刀菌、西瓜尖孢镰刀菌和番茄尖孢镰刀菌的生长，其抑菌圈分别为39.9、33.7、30.8、19.9和22.5毫米。