Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Aug;79(3):631-7. doi: 10.1016/j.saa.2011.03.046. Epub 2011 Apr 8.
In a weak alkaline Britton-Robinson buffer medium, erythrosine (Ery) can react with Fe(phen)(3)(2+) to form 1:1 ion-association complex, which will cause not only the changes of the absorption spectra, but also the remarkable enhancement of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) spectra, and the appearance of new spectra of RRS, SOS and FDS. The maximum RRS, SOS and FDS wavelengths (λ(ex)/λ(em)) of the ion-association complex are located at 358/358 nm, 290/580 nm and 780/390 nm, respectively. The increments of scattering intensities (ΔI) are directly proportional to the concentration of Ery in a certain range. The detection limits for Ery are 0.028 μg mL(-1) for RRS method, 0.068 μg mL(-1) for SOS method and 0.11 μg mL(-1) for FDS method, respectively. Among them, the RRS method has the highest sensitivity. Based on the above researches, a new highly sensitive and simple method for the determination of Ery has been developed. In this work, the spectral characteristics of absorption, RRS, SOS and FDS spectra, the optimum conditions of the reaction and influencing factors for the RRS, SOS and FDS intensities were investigated. In addition, the reaction mechanism was discussed.
在弱碱性 Britton-Robinson 缓冲介质中，赤藓红（Ery）可以与 Fe(phen)(3)(2+)反应形成 1:1 的离子缔合物，这不仅会导致吸收光谱的变化，还会显著增强共振瑞利散射（RRS）、二级散射（SOS）和倍频散射（FDS）光谱，并出现新的 RRS、SOS 和 FDS 光谱。离子缔合物的最大 RRS、SOS 和 FDS 波长（λ(ex)/λ(em)）分别位于 358/358nm、290/580nm 和 780/390nm。散射强度的增量（ΔI）与一定范围内 Ery 的浓度成正比。RRS 法、SOS 法和 FDS 法测定 Ery 的检出限分别为 0.028μgmL(-1)、0.068μgmL(-1)和 0.11μgmL(-1)。其中，RRS 法具有最高的灵敏度。基于上述研究，建立了一种测定 Ery 的高灵敏度、简单方法。本工作研究了吸收光谱、RRS、SOS 和 FDS 光谱的光谱特征、反应的最佳条件以及 RRS、SOS 和 FDS 强度的影响因素。此外，还探讨了反应机理。
