Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
Parasitol Res. 2011 Oct;109(4):1035-43. doi: 10.1007/s00436-011-2341-4. Epub 2011 May 4.
Sheep infected with the Cullompton isolate of Fasciola hepatica were treated with triclabendazole at a concentration of 10 mg/kg at 12 weeks post-infection. Adult flukes were recovered from the liver and, where present, from the gall bladder at 48, 72 and 96 h post-treatment (pt). Gross changes to the spermatogenic cells of the testis were examined by histology and ultrastructural alterations were visualised via transmission electron microscopy. Disruption was progressive in nature, with the testis tubules becoming shrunken, vacuolated and gradually more denuded of cellular content over the 96-h time period. From 48 h pt, the number of primary and secondary spermatogonia decreased and multinucleate spermatogonial cells were frequent. Later, developmental stages were uncommon, giving rise to much empty space within the tubules. By 72 h pt, the tubules contained many apoptotic and degraded cells and had an extremely disorganised appearance. At 96 h pt, the tubules were almost completely empty, with the exception of the remains of degraded spermatogenic cells. These results indicate that triclabendazole severely disrupts spermatogenesis in the liver fluke from 48 h pt in vivo.
感染了库伦顿（Cullompton）利什曼原虫分离株的绵羊在感染后 12 周，以 10mg/kg 的浓度用三氯苯达唑进行治疗。成虫在治疗后 48、72 和 96 小时（pt）从肝脏和（如果存在的话）胆囊中回收。通过组织学检查观察到睾丸精子发生细胞的明显变化，通过透射电子显微镜观察到超微结构改变。这种破坏是渐进性的，睾丸小管逐渐缩小、空泡化，并逐渐失去更多的细胞内容物。从 48 h pt 开始，初级和次级精原细胞的数量减少，多核精原细胞频繁出现。后来，发育阶段很少见，导致小管内出现大量空洞。到 72 h pt 时，小管内含有许多凋亡和退化的细胞，外观极不规则。到 96 h pt 时，小管几乎完全空了，除了退化的精子发生细胞的残余物。这些结果表明，三氯苯达唑在体内从 48 h pt 开始严重破坏肝吸虫的精子发生。
